Short transient receptor potential channel 1 (TrpC1), Goat Polyclonal Antibody

Product Info

Product Name Short transient receptor potential channel 1 (TrpC1), Goat Polyclonal Antibody
Product Description google Goat anti-Short transient receptor potential channel 1 (TrpC1) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, ICC, FC, IHC.
Alternative Names TRPC1; transient receptor potential cation channel, subfamily C, member 1; HTRP-1; MGC133334; MGC133335; TRP1; transient receptor potential channel 1
Application(s) FC, ICC, IHC, Pep-ELISA
Antibody Host Goat
Antibody Type Polyclonal
Specificity Reacts with TRPC1 from Human.
Species Reactivity Dog, Human, Mouse, Rat
Immunogen Description A synthetic peptide consisting of amino acids, C-NLKQKRDENYQK
Conjugate Unconjugated
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Regulatory Status For research use only.

Specifications

Product Description Goat anti-Short transient receptor potential channel 1 (TrpC1) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, ICC, FC, IHC.
Application(s) FC, ICC, IHC, Pep-ELISA
Application Details Peptide ELISA (1:32000), Immunohistochemistry (3.75 µg/mL) Immunocytochemistry (10 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Short transient receptor potential channel 1 (TrpC1)
Specificity Reacts with TRPC1 from Human.
Target Host Species Human
Species Reactivity Dog, Human, Mouse, Rat
Antibody Host Goat
Antibody Type Polyclonal
Antibody Isotype IgG
Conjugate Unconjugated
Immunogen Description A synthetic peptide consisting of amino acids, C-NLKQKRDENYQK
Sequence NLKQKRDENYQK
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names TRPC1; transient receptor potential cation channel, subfamily C, member 1; HTRP-1; MGC133334; MGC133335; TRP1; transient receptor potential channel 1
Uniprot Number P48995
Uniprot Number/Name P48995 (TRPC1_HUMAN)
Scientific Background Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Seems to be also activated by intracellular calcium store depletion.
Shipping Temperature 2-8°C (on cold packs)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

A. Immunofluorescence analysis: of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (4 µg/mL), showing cytoplasmic/Vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (4 µg/mL). B. Immunofluorescence analysis: of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (4 µg/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (4 µg/mL).
Human Cortex Staining: (3.75 µg/mL) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

A. Flow cytometric analysis: of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5%Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. B. Human Prostate Staining: (3.75 µg/mL) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Citations & References