Short transient receptor potential channel 6 (TrpC6), Goat Polyclonal Antibody

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Catalog Number
G-1878

    Product Info

  • Product Name Short transient receptor potential channel 6 (TrpC6), Goat Polyclonal Antibody
  • Product Description google Goat anti-Short transient receptor potential channel 6 (TrpC6) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, FC.
  • Alternative Names TRPC6; transient receptor potential cation channel, subfamily C, member 6; HGNC:12338; TRP6; transient receptor potential channel 6; FLJ11098; FLJ14863; FSGS2; TRP6
  • Application(s) FC, WB, Pep-ELISA
  • Antibody Host Goat
  • Antibody Type Polyclonal
  • Specificity Reacts with TRPC6 from Human.
  • Species Reactivity Bovine (Predicted), Dog (Predicted), Human, Pig (Predicted)
  • Immunogen Description A synthetic peptide consisting of amino acids, C-EKLSMEPNQEETN
  • Conjugate Unconjugated
  • Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Regulatory Status For research use only.

    Specifications

  • Product Description Goat anti-Short transient receptor potential channel 6 (TrpC6) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, FC.
  • Application(s) FC, WB, Pep-ELISA
  • Application Details Peptide ELISA (1:2000), Western Blot (0.3-1 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Short transient receptor potential channel 6 (TrpC6)
  • Specificity Reacts with TRPC6 from Human.
  • Target Host Species Human
  • Species Reactivity Bovine (Predicted), Dog (Predicted), Human, Pig (Predicted)
  • Antibody Host Goat
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide consisting of amino acids, C-EKLSMEPNQEETN
  • Sequence EKLSMEPNQEETN
  • Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
  • Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names TRPC6; transient receptor potential cation channel, subfamily C, member 6; HGNC:12338; TRP6; transient receptor potential channel 6; FLJ11098; FLJ14863; FSGS2; TRP6
  • Uniprot Number Q9Y210
  • Uniprot Number/Name Q9Y210 (TRPC6_HUMAN)
  • Scientific Background Thought to form a receptor-activated non-selective calcium permeant cation channel (PubMed:19936226, PubMed:23291369). Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C (PubMed:26892346). Seems not to be activated by intracellular calcium store depletion (uniprot).
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • A. Western Blot: (1 µg/mL) staining of A549 cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence. B. Flow cytometry analysis: of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

    Citations & References