Product NameSonic hedgehog protein (SHH), Goat Polyclonal Antibody
Product DescriptiongoogleGoat anti-Sonic hedgehog protein (SHH) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, ICC, FC.
Alternative NamesSHH; sonic hedgehog homolog (Drosophila); HGNC:10848; HHG1; HLP3; HPE3; SMMCI ; sonic hedgehog; ShhNC;
Application(s)FC, ICC, WB, Pep-ELISA
Antibody HostGoat
Antibody TypePolyclonal
SpecificityReacts with SHH from Human, Mouse.
Species ReactivityDog, Human, Mouse, Rat
Immunogen DescriptionA synthetic peptide consisting of amino acids, C-DSEALHPLGMAVK
ConjugateUnconjugated
Purity DescriptionPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Product DescriptionGoat anti-Sonic hedgehog protein (SHH) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, ICC, FC.
Application(s)FC, ICC, WB, Pep-ELISA
Application DetailsPeptide ELISA (1:32,000), Western Blot (1-3 µg/mL), Immunocytochemistry (10 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetSonic hedgehog protein (SHH)
SpecificityReacts with SHH from Human, Mouse.
Target Host SpeciesHuman
Species ReactivityDog, Human, Mouse, Rat
Antibody HostGoat
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide consisting of amino acids, C-DSEALHPLGMAVK
SequenceDSEALHPLGMAVK
Purity DescriptionPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
FormatLiquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage InstructionsUpon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesSHH; sonic hedgehog homolog (Drosophila); HGNC:10848; HHG1; HLP3; HPE3; SMMCI ; sonic hedgehog; ShhNC;
Scientific BackgroundSonic hedgehog protein: The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (By similarity). Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN (By similarity). Both activities occur in the reticulum endoplasmic (By similarity). Once cleaved, ShhC is degraded in the endoplasmic reticulum
A. Western Blot analysis : (2 µg/ml) staining of Human Liver lysate (35μg protein in RIPA buffer). Detected by chemiluminescence. B. Immunofluorescence analysis: of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
A. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL). B. Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
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