Product NameSuppressor of cytokine signaling 1 (SOCS1), Goat Polyclonal Antibody
Product DescriptiongoogleGoat anti-Suppressor of cytokine signaling 1 (SOCS1) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, FC, ICC, IHC.
Alternative NamesSOCS1; JAB; CIS1; SSI1; TIP3; CISH1; Cish1; SSI-1; SOCS-1; suppressor of cytokine signaling 1; JAK binding protein; Tec-interacting protein 3; STAT induced SH3 protein 1; cytokine-inducible SH2 protein 1
Application(s)FC, ICC, IHC, Pep-ELISA
Antibody HostGoat
Antibody TypePolyclonal
SpecificityReacts with SOCS1 from Human, Mouse.
Species ReactivityBovine (Predicted), Dog (Predicted), Human, Mouse, Pig, Rat (Predicted)
Immunogen DescriptionA synthetic peptide consisting of amino acids, C-VLRDYLSSFPFQI
ConjugateUnconjugated
Purity DescriptionPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Product DescriptionGoat anti-Suppressor of cytokine signaling 1 (SOCS1) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, FC, ICC, IHC.
Application(s)FC, ICC, IHC, Pep-ELISA
Application DetailsELISA (1:32000), Western Blotting (0.1-1 µg/mL), Immunocytochemistry (5 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetSuppressor of cytokine signaling 1 (SOCS1)
SpecificityReacts with SOCS1 from Human, Mouse.
Target Host SpeciesHuman
Species ReactivityBovine (Predicted), Dog (Predicted), Human, Mouse, Pig, Rat (Predicted)
Antibody HostGoat
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide consisting of amino acids, C-VLRDYLSSFPFQI
SequenceVLRDYLSSFPFQI
Purity DescriptionPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
FormatLiquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage InstructionsUpon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesSOCS1; JAB; CIS1; SSI1; TIP3; CISH1; Cish1; SSI-1; SOCS-1; suppressor of cytokine signaling 1; JAK binding protein; Tec-interacting protein 3; STAT induced SH3 protein 1; cytokine-inducible SH2 protein 1
Scientific BackgroundSOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway (Uniprot)
A. Flow cytometry analysis: paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. B. Western Blot: (0.3 µg/mL) staining of Human Spleen (A), Pig Pancreas (B), (1 µg/mL) Mouse Spleen (C) lysate and negative control HeLa (D) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
A. Staining Human Kidney: (5 µg/mL) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining. B. Staining Human Kidney: Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
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