Transcription intermediary factor 1-beta (TIF1-beta), Goat Polyclonal Antibody

Product Info

Product Name Transcription intermediary factor 1-beta (TIF1-beta), Goat Polyclonal Antibody
Product Description google Goat anti-Transcription intermediary factor 1-beta (TIF1-beta) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, ICC, FC, IHC.
Alternative Names TRIM28; KAP1; TF1B; RNF96; TIF1B; tripartite motif-containing 28; KRAB-associated protein 1; nuclear corepressor KAP-1; transcriptional intermediary factor 1-beta; FLJ29029; KRAB-associated protein 1; tripartite motif-containing 28 protein
Application(s) FC, ICC, IHC, Pep-ELISA
Antibody Host Goat
Antibody Type Polyclonal
Specificity Reacts with KAP1 / TRIM28 from Human.
Species Reactivity Human, Mouse (Predicted)
Immunogen Description A synthetic peptide consisting of amino acids, C-SSQELSGGPGDGP
Conjugate Unconjugated
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Regulatory Status For research use only.

Specifications

Product Description Goat anti-Transcription intermediary factor 1-beta (TIF1-beta) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, ICC, FC, IHC.
Application(s) FC, ICC, IHC, Pep-ELISA
Application Details Peptide ELISA (1:2000), Immunohistochemistry (2-4 µg/mL), Immunofluorescence (10 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Transcription intermediary factor 1-beta (TIF1-beta)
Specificity Reacts with KAP1 / TRIM28 from Human.
Target Host Species Human
Species Reactivity Human, Mouse (Predicted)
Antibody Host Goat
Antibody Type Polyclonal
Antibody Isotype IgG
Conjugate Unconjugated
Immunogen Description A synthetic peptide consisting of amino acids, C-SSQELSGGPGDGP
Sequence SSQELSGGPGDGP
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names TRIM28; KAP1; TF1B; RNF96; TIF1B; tripartite motif-containing 28; KRAB-associated protein 1; nuclear corepressor KAP-1; transcriptional intermediary factor 1-beta; FLJ29029; KRAB-associated protein 1; tripartite motif-containing 28 protein
Uniprot Number Q13263
Uniprot Number/Name Q13263 (TIF1B_HUMAN)
Scientific Background The protein encoded by TRIM28 gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]
Shipping Temperature 2-8°C (on cold packs)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

A. Flow cytometry analysis: paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. B. Staining Human Breast Tissue: (2 µg/mL) staining of paraffin embedded Human Breast. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
A. Immunofluorescence analysis: paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL). B. Immunofluorescence analysis: paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Citations & References