Transmembrane emp24 domain-containing protein 10 (TMED10), Goat Polyclonal Antibody

Product Info

Product Name Transmembrane emp24 domain-containing protein 10 (TMED10), Goat Polyclonal Antibody
Product Description google Goat anti-Transmembrane emp24 domain-containing protein 10 (TMED10) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, ICC, FC.
Alternative Names TMED10; transmembrane emp24-like trafficking protein 10 (yeast); P24(DELTA); S31I125; S31III125; TMP21; Tmp-21-I ; 21 kDa transmembrane trafficking protein; transmembrane emp24 domain-containing protein 10
Application(s) FC, ICC, Pep-ELISA
Antibody Host Goat
Antibody Type Polyclonal
Specificity Reacts with TMP21 / TMED10 from Human.
Species Reactivity Bovine (Predicted), Dog (Predicted), Human, Mouse (Predicted), Rat (Predicted)
Immunogen Description A synthetic peptide consisting of amino acids, C-YLRRFFKAKKLIE
Conjugate Unconjugated
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Regulatory Status For research use only.

Specifications

Product Description Goat anti-Transmembrane emp24 domain-containing protein 10 (TMED10) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, ICC, FC.
Application(s) FC, ICC, Pep-ELISA
Application Details Peptide ELISA (1:1000), Immunofluorescence (10 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Transmembrane emp24 domain-containing protein 10 (TMED10)
Specificity Reacts with TMP21 / TMED10 from Human.
Target Host Species Human
Species Reactivity Bovine (Predicted), Dog (Predicted), Human, Mouse (Predicted), Rat (Predicted)
Antibody Host Goat
Antibody Type Polyclonal
Antibody Isotype IgG
Conjugate Unconjugated
Immunogen Description A synthetic peptide consisting of amino acids, C-YLRRFFKAKKLIE
Sequence YLRRFFKAKKLIE
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names TMED10; transmembrane emp24-like trafficking protein 10 (yeast); P24(DELTA); S31I125; S31III125; TMP21; Tmp-21-I ; 21 kDa transmembrane trafficking protein; transmembrane emp24 domain-containing protein 10
Uniprot Number P49755
Uniprot Number/Name P49755 (TMEDA_HUMAN)
Scientific Background Involved in vesicular protein trafficking. Mainly functions in the early secretory pathway. Thought to act as cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and to be involved in vesicle coat formation at the cytoplasmic side Uniprot).
Shipping Temperature 2-8°C (on cold packs)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

A. Immunofluorescence analysis: of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing Golgi/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL). B. Flow cytometry analysis: of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Citations & References