Type II iodothyronine deiodinase (DIO2), Goat Polyclonal Antibody

Product Info

Product Name Type II iodothyronine deiodinase (DIO2), Goat Polyclonal Antibody
Product Description google Goat anti-Type II iodothyronine deiodinase (DIO2) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, FC, ICC, IHC.
Alternative Names DIO2; deiodinase, iodothyronine, type II ; 5DII; D2; SelY; TXDI2 ; thyroxine deiodinase, type II; type 2 iodothyronine deiodinase; type II iodothyronine deiodinaseN; type-II 5'deiodinase;
Application(s) FC, ICC, IHC, Pep-ELISA
Antibody Host Goat
Antibody Type Polyclonal
Specificity Reacts with DIO2 from Human, Mouse. This antibody is expected to recognise both reported isoform a (NP_000784.2 and NP_054644.1) and isoform b (NP_001007024.1).
Species Reactivity Human, Mouse, Pig (Predicted), Rat (Predicted)
Immunogen Description A synthetic peptide consisting of amino acids, EVKKHQNQEDRC
Conjugate Unconjugated
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Regulatory Status For research use only.

Specifications

Product Description Goat anti-Type II iodothyronine deiodinase (DIO2) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, FC, ICC, IHC.
Application(s) FC, ICC, IHC, Pep-ELISA
Application Details Peptide ELISA (1:128000), Immunohistochemistry (2-3 µg/mL), Immunofluorescence (10 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Type II iodothyronine deiodinase (DIO2)
Specificity Reacts with DIO2 from Human, Mouse. This antibody is expected to recognise both reported isoform a (NP_000784.2 and NP_054644.1) and isoform b (NP_001007024.1).
Target Host Species Human
Species Reactivity Human, Mouse, Pig (Predicted), Rat (Predicted)
Antibody Host Goat
Antibody Type Polyclonal
Antibody Isotype IgG
Conjugate Unconjugated
Immunogen Description A synthetic peptide consisting of amino acids, EVKKHQNQEDRC
Sequence EVKKHQNQEDRC
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names DIO2; deiodinase, iodothyronine, type II ; 5DII; D2; SelY; TXDI2 ; thyroxine deiodinase, type II; type 2 iodothyronine deiodinase; type II iodothyronine deiodinaseN; type-II 5'deiodinase;
Uniprot Number Q92813
Uniprot Number/Name Q92813 (IOD2_HUMAN)
Scientific Background Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development.
Shipping Temperature 2-8°C (on cold packs)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

A. Flow cytometry analysis: of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. B. Immunofluorescence analysis: of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
G-1920-100 (2 µg/mL) staining of PFA-fixed cryo-sectioned Mouse Hippocampus. Microwaved antigen retrieval with citrate buffer pH 4.5, HRP-staining.

Citations & References