Ubiquitin carboxyl-terminal hydrolase 6 (USP6), Goat Polyclonal Antibody

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Catalog Number
G-1907

    Product Info

  • Product Name Ubiquitin carboxyl-terminal hydrolase 6 (USP6), Goat Polyclonal Antibody
  • Product Description google Goat anti-Ubiquitin carboxyl-terminal hydrolase 6 (USP6) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, ICC, FC.
  • Alternative Names USP6; ubiquitin specific peptidase 6 (Tre-2 oncogene); HRP1; TRE17; TRE2; Tre-2; USP6-short; OTTHUMP00000125301; deubiquitinating enzyme 6; hyperpolymorphic gene 1; proto-oncogene TRE-2; tre-2 oncogene; ubiquitin carboxyl-terminal hydrolase 6; ubiquitin specific peptidase 6-; ubiquitin specific protease 6 (Tre-2 oncogene); ubiquitin thioesterase 6; ubiquitin thiolesterase 6; ubiquitin-specific protease USP6; ubiquitin-specific-processing protease 6
  • Application(s) FC, ICC, Pep-ELISA
  • Antibody Host Goat
  • Antibody Type Polyclonal
  • Specificity Reacts with USP6 (aa142-155) from Human. This antibody is expected NOT to cross react to USP32 or to the TBC1D3 proteins.
  • Species Reactivity Human
  • Immunogen Description A synthetic peptide consisting of amino acids, C-HHIDLDVRTTLRNH
  • Conjugate Unconjugated
  • Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Regulatory Status For research use only.

    Specifications

  • Product Description Goat anti-Ubiquitin carboxyl-terminal hydrolase 6 (USP6) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, ICC, FC.
  • Application(s) FC, ICC, Pep-ELISA
  • Application Details Peptide ELISA (1:4000),Western Blot (1 µg/mL), Immunocytochemistry (10 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Ubiquitin carboxyl-terminal hydrolase 6 (USP6)
  • Specificity Reacts with USP6 (aa142-155) from Human. This antibody is expected NOT to cross react to USP32 or to the TBC1D3 proteins.
  • Target Host Species Human
  • Species Reactivity Human
  • Antibody Host Goat
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide consisting of amino acids, C-HHIDLDVRTTLRNH
  • Sequence HHIDLDVRTTLRNH
  • Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
  • Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names USP6; ubiquitin specific peptidase 6 (Tre-2 oncogene); HRP1; TRE17; TRE2; Tre-2; USP6-short; OTTHUMP00000125301; deubiquitinating enzyme 6; hyperpolymorphic gene 1; proto-oncogene TRE-2; tre-2 oncogene; ubiquitin carboxyl-terminal hydrolase 6; ubiquitin specific peptidase 6-; ubiquitin specific protease 6 (Tre-2 oncogene); ubiquitin thioesterase 6; ubiquitin thiolesterase 6; ubiquitin-specific protease USP6; ubiquitin-specific-processing protease 6
  • Uniprot Number P35125
  • Uniprot Number/Name P35125 (UBP6_HUMAN)
  • Scientific Background Deubiquitinase with an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety. Catalyzes its own deubiquitination. In vitro, isoform 2, but not isoform 3, shows deubiquitinating activity. Promotes plasma membrane localization of ARF6 and selectively regulates ARF6-dependent endocytic protein trafficking. Is able to initiate tumorigenesis by inducing the production of matrix metalloproteinases following NF-kappa-B activation
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • A. Immunofluorescence analysis: of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic/vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL). B. Immunofluorescence analysis: of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

  • Flow cytometric analysis: of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

    Citations & References