SpecificityReacts with USP6 (aa142-155) from Human. This antibody is expected NOT to cross react to USP32 or to the TBC1D3 proteins.
Species ReactivityHuman
Immunogen DescriptionA synthetic peptide consisting of amino acids, C-HHIDLDVRTTLRNH
ConjugateUnconjugated
Purity DescriptionPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Product DescriptionGoat anti-Ubiquitin carboxyl-terminal hydrolase 6 (USP6) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, ICC, FC.
Application(s)FC, ICC, Pep-ELISA
Application DetailsPeptide ELISA (1:4000),Western Blot (1 µg/mL), Immunocytochemistry (10 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityReacts with USP6 (aa142-155) from Human. This antibody is expected NOT to cross react to USP32 or to the TBC1D3 proteins.
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostGoat
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide consisting of amino acids, C-HHIDLDVRTTLRNH
SequenceHHIDLDVRTTLRNH
Purity DescriptionPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
FormatLiquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage InstructionsUpon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundDeubiquitinase with an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety. Catalyzes its own deubiquitination. In vitro, isoform 2, but not isoform 3, shows deubiquitinating activity. Promotes plasma membrane localization of ARF6 and selectively regulates ARF6-dependent endocytic protein trafficking. Is able to initiate tumorigenesis by inducing the production of matrix metalloproteinases following NF-kappa-B activation