SpecificityReacts with UBE2C / UBCH10 from Human. This antibody is expected to recognise all reported isoforms. Variants NP_861517.1 and NP_861518.1 encode the same isoform.
Species ReactivityHuman
Immunogen DescriptionA synthetic peptide consisting of amino acids, C-QETYSKQVTSQEP
ConjugateUnconjugated
Purity DescriptionPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Product DescriptionGoat anti-Ubiquitin-conjugating enzyme E2 C (UBE2C) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, ICC, FC, IP.
Application(s)FC, ICC, IP, WB, Pep-ELISA
Application DetailsPeptide ELISA (1:32000), Western Blot (1-3 µg/mL), Immunofluorescence (10 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetUbiquitin-conjugating enzyme E2 C (UBE2C)
SpecificityReacts with UBE2C / UBCH10 from Human. This antibody is expected to recognise all reported isoforms. Variants NP_861517.1 and NP_861518.1 encode the same isoform.
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostGoat
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide consisting of amino acids, C-QETYSKQVTSQEP
SequenceQETYSKQVTSQEP
Purity DescriptionPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
FormatLiquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage InstructionsUpon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit (Ref. Uniprot)
A.Western Blot: (2 µg/mL) staining of HEK293 (A) and HeLa (B) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence. B. Flow cytometry analysis: paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
A. Immunofluorescence analysis: paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (4 µg/mL), showing cytoplasmic /Plasma Membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (4 µg/mL). B. Immunofluorescence analysis: paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (4 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (4 µg/mL).
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