Ubiquitin-conjugating enzyme E2 S (UBE2S), Goat Polyclonal Antibody

Product Info

Product Name Ubiquitin-conjugating enzyme E2 S (UBE2S), Goat Polyclonal Antibody
Product Description google Goat anti-Ubiquitin-conjugating enzyme E2 S (UBE2S) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, ICC, FC.
Alternative Names E2-EPF; ubiquitin carrier protein; ubiquitin-protein ligase; ubiquitin-conjugating enzyme E2-24 kD; UBE2S; ubiquitin-conjugating enzyme E2S; E2EPF; EPF5; ubiquitin carrier protein S; ubiquitin-protein ligase S
Application(s) FC, ICC, Pep-ELISA
Antibody Host Goat
Antibody Type Polyclonal
Specificity Reacts with E2-EPF / UBE2S from Human.
Species Reactivity Bovine (Predicted), Dog (Predicted), Human, Mouse (Predicted), Rat (Predicted)
Immunogen Description A synthetic peptide consisting of amino acids, NSNVENLPPHIIR-C
Conjugate Unconjugated
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Regulatory Status For research use only.

Specifications

Product Description Goat anti-Ubiquitin-conjugating enzyme E2 S (UBE2S) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, ICC, FC.
Application(s) FC, ICC, Pep-ELISA
Application Details Peptide ELISA (1:32000), Immunofluorescence (10 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Ubiquitin-conjugating enzyme E2 S (UBE2S)
Specificity Reacts with E2-EPF / UBE2S from Human.
Target Host Species Human
Species Reactivity Bovine (Predicted), Dog (Predicted), Human, Mouse (Predicted), Rat (Predicted)
Antibody Host Goat
Antibody Type Polyclonal
Antibody Isotype IgG
Conjugate Unconjugated
Immunogen Description A synthetic peptide consisting of amino acids, NSNVENLPPHIIR-C
Sequence NSNVENLPPHIIR
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names E2-EPF; ubiquitin carrier protein; ubiquitin-protein ligase; ubiquitin-conjugating enzyme E2-24 kD; UBE2S; ubiquitin-conjugating enzyme E2S; E2EPF; EPF5; ubiquitin carrier protein S; ubiquitin-protein ligase S
Uniprot Number Q16763
Uniprot Number/Name Q16763 (UBE2S_HUMAN)
Scientific Background The gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. [provided by RefSeq, Jul 2008]
Shipping Temperature 2-8°C (on cold packs)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

A. Immunofluorescence analysis: paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL). B. Immunofluorescence analysis: paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL) C. Flow cytometry analysis: paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Citations & References