Zinc finger protein 423 (ZNF423), Goat Polyclonal Antibody

Product Info

Product Name Zinc finger protein 423 (ZNF423), Goat Polyclonal Antibody
Product Description google Goat anti-Zinc finger protein 423 (ZNF423) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, ICC, FC.
Alternative Names ZNF423 ; zinc finger protein 423 ; Ebfaz; KIAA0760; MGC138520; MGC138522; OAZ; Roaz; ZFP423; Zfp104; OLF-1/EBF associated zinc finger; Smad- and Olf-interacting zinc finger protein; early B-cell factor associated zinc finger protein; hOAZ;
Application(s) FC, ICC, Pep-ELISA
Antibody Host Goat
Antibody Type Polyclonal
Specificity Reacts with OAZ from Human.
Species Reactivity Human, Mouse (Predicted)
Immunogen Description A synthetic peptide consisting of amino acids, EPECDQKTSRALEDR
Conjugate Unconjugated
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Regulatory Status For research use only.

Specifications

Product Description Goat anti-Zinc finger protein 423 (ZNF423) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, ICC, FC.
Application(s) FC, ICC, Pep-ELISA
Application Details Peptide ELISA (1:2000), Immunofluorescence ( 10 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Zinc finger protein 423 (ZNF423)
Specificity Reacts with OAZ from Human.
Target Host Species Human
Species Reactivity Human, Mouse (Predicted)
Antibody Host Goat
Antibody Type Polyclonal
Antibody Isotype IgG
Conjugate Unconjugated
Immunogen Description A synthetic peptide consisting of amino acids, EPECDQKTSRALEDR
Sequence EPECDQKTSRALEDR
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names ZNF423 ; zinc finger protein 423 ; Ebfaz; KIAA0760; MGC138520; MGC138522; OAZ; Roaz; ZFP423; Zfp104; OLF-1/EBF associated zinc finger; Smad- and Olf-interacting zinc finger protein; early B-cell factor associated zinc finger protein; hOAZ;
Uniprot Number Q2M1K9
Uniprot Number/Name Q2M1K9 (ZN423_HUMAN)
Scientific Background Transcription factor that can both act as an activator or a repressor depending on the context. Plays a central role in BMP signaling and olfactory neurogenesis. Associates with SMADs in response to BMP2 leading to activate transcription of BMP target genes. Acts as a transcriptional repressor via its interaction with EBF1, a transcription factor involved in terminal olfactory receptor neurons differentiation; this interaction preventing EBF1 to bind DNA and activate olfactory-specific genes. Involved in olfactory neurogenesis by participating in a developmental switch that regulates the transition from differentiation to maturation in olfactory receptor neurons. Controls proliferation and differentiation of neural precursors in cerebellar vermis formation (Uniprot).
Shipping Temperature 2-8°C (on cold packs)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

A. Immunofluorescence analysis: of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL). B. Flow cytometry analysis: of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Citations & References