SpecificityThe specificity of this antibody was demonstrated by WB and ELISA against the GST tagged recombinant Epidermal growth factor-like 1 protein. This antibody has not been tested against other species.
Species ReactivityHuman
Immunogen DescriptionA partial recombinant protein of human Epidermal growth factor-like 1 (amino acids 71 to 180) with a GST tag.
ConjugateUnconjugated
Purity DescriptionProtein G purified immunoglobulin
Application DetailsThis antibody is recommended for WB and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetEpidermal growth factor-like 1
SpecificityThe specificity of this antibody was demonstrated by WB and ELISA against the GST tagged recombinant Epidermal growth factor-like 1 protein. This antibody has not been tested against other species.
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG
Clone Name2F7
ConjugateUnconjugated
Immunogen DescriptionA partial recombinant protein of human Epidermal growth factor-like 1 (amino acids 71 to 180) with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.2
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Epidermal growth factor-like 1 belongs to the G-protein coupled receptor 2 family and the LN-TM7 subfamily. The unprocessed precursor has a length of 3312 amino acids and a predicted molecular weight of 358.3 KDa. It contains nine cadherin domains, eight EGF-like domains, one GPS domains, one laminin EGF-like domains and two laminin G-like domains. It is a transmembrane protein and is thought to have an important role in cell to cell signaling during nervous system formation.
Western blot analysis on recombinant GST tagged Epidermal growth factor-like 1 protein using mouse monoclonal antibody to human Epidermal growth factor-like 1 [2F7], catalogue number M-812-100.
In sandwhich ELISA, mouse monoclonal antibody to human CELR3 [2F7], catalogue number M-812-100, has a detection limit of 0.03ng/mL for the GST tagged recombinant CELR3 protein.