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Muscarinic acetylcholine receptor M2, Mouse Monoclonal Antibody
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Catalog Number
M-814
- Product Name Muscarinic acetylcholine receptor M2, Mouse Monoclonal Antibody
- Product Description Mouse anti-Muscarinic acetylcholine receptor M2 Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
- Alternative Names CHRM2
- Application(s) ELISA, WB
- Antibody Host Mouse
- Antibody Type Monoclonal
- Specificity The reactivity of this antibody has been confirmed by WB and ELISA against the recombinant GST tagged muscarinic acetylcholine receptor protein. This antibody has not yet been tested against other species.
- Species Reactivity Human
- Immunogen Description Partial recombinant protein of human muscarinic acetylcholine receptor (amino acids 251 to 350) with a GST tag.
- Conjugate Unconjugated
- Purity Description Protein G purified immunoglobulin
- Regulatory Status For research use only.
Product Info
- Product Description Mouse anti-Muscarinic acetylcholine receptor M2 Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
- Application(s) ELISA, WB
- Application Details This antibody is recommended for WB, direct and sandwich ELISA. The detection limit of recombinant GST tagged muscarinic acetylcholine receptor is 0.03ng/mL when using mouse monoclonal antibody to recombinant GST muscarinic acetylcholine receptor [4B5], catalogue number M-814-100, as a capture antibody. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- Target Muscarinic acetylcholine receptor M2
- Specificity The reactivity of this antibody has been confirmed by WB and ELISA against the recombinant GST tagged muscarinic acetylcholine receptor protein. This antibody has not yet been tested against other species.
- Target Host Species Human
- Species Reactivity Human
- Antibody Host Mouse
- Antibody Type Monoclonal
- Antibody Isotype IgG2a, kappa
- Clone Name 4B5
- Conjugate Unconjugated
- Immunogen Description Partial recombinant protein of human muscarinic acetylcholine receptor (amino acids 251 to 350) with a GST tag.
- Purity Description Protein G purified immunoglobulin
- Format Lyophilized from PBS pH 7.4
- Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
- Storage Instructions Store lyophilized antibody at 2-8ºC. After reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names CHRM2
- Uniprot Number P08172
- Uniprot Number/Name P08172 (ACM2_HUMAN
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Scientific Background
The muscarinic acetylcholine receptor belongs to the G-protein coupled receptor 1 family and mediates various cellular responses such as adenylate cyclase inhibition, phosphoinositide breakdown and potassium channel modulation through the action of G proteins. The unprocessed precursor has a length of 466 amino acids and a predicted molecular weight of 51.7KDa.
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.
