SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; predicted to react with mouse due to sequence homology
Species ReactivityHuman, Mouse (Predicted), Rat
Immunogen DescriptionA synthetic peptide (FAFYKKARSMTDVYLLN) corresponding to a region (71-87) from human C-C Chemokine Receptor type 6. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
ConjugateUnconjugated
Purity DescriptionAffinity purified on antigen column
Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.75 µg/mL is recommended for WB. Human CCR6 has a predicted length of 374 residues and MW of 43 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetC-C Chemokine Receptor type 6 (C-C CKR-6)
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; predicted to react with mouse due to sequence homology
Target Host SpeciesHuman
Species ReactivityHuman, Mouse (Predicted), Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide (FAFYKKARSMTDVYLLN) corresponding to a region (71-87) from human C-C Chemokine Receptor type 6. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
Purity DescriptionAffinity purified on antigen column
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundC-C Chemokine Receptor type 6 (CCR6) is a multi-pass membrane protein and G-protein coupled receptor for chemokines such as MIP-3 alpha. CCR6 is expressed in lymphocytes, T-cells and B-cells.
Immunohistochemical staining of paraffin-embedded human large intestine cancer sections using rabbit polyclonal antibody to human C-C Chemokine Receptor type 6 (0.5-1 µg/ml). Secondary biotinylated goat anti-rabbit IgG antibody (10 µg/ml).