Glial fibrillary acidic protein (GFAP), Chicken Polyclonal Antibody

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Catalog Number
C-1373

    Product Info

  • Product Name Glial fibrillary acidic protein (GFAP), Chicken Polyclonal Antibody
  • Product Description google
    Chicken anti-Glial fibrillary acidic protein (GFAP) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Paraffin-embedded, IHC-Frozen, ICC.
  • Alternative Names Astrocyte; Glial fibrillary acidic protein; GFAP;
  • Application(s) ICC, IHC-Frozen, IHC-Paraffin-embedded, WB
  • Antibody Host Chicken
  • Antibody Type Polyclonal
  • Specificity The specificity of this antibody has been confirmed by WB. Human, Rat, Mouse, Feline. Predicted to react with other mammals.
  • Species Reactivity Cat, Human, Mouse, Other Mammals (Predicted), Rat
  • Immunogen Description Recombinant GFAP (expressed in E.coli) and native bovine GFAP
  • Conjugate Unconjugated
  • Purity Description IgY
  • Regulatory Status For research use only.

    Specifications

  • Product Description
    Chicken anti-Glial fibrillary acidic protein (GFAP) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Paraffin-embedded, IHC-Frozen, ICC.
  • Application(s) ICC, IHC-Frozen, IHC-Paraffin-embedded, WB
  • Application Details Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). WB: A dilution of 1:5,000 is recommended. ICC: A dilution of 1:1,000-1:5,000 using fluorescent secondary antibodies or peroxidase or other enzyme-linked methods is recommended on 4% PFA fixed cells in culture, with 3hr-o/n incubation of primary antibody. IHC: 4% PFA frozen tissues, permeabilized. IHC (paraffin-embedded): capable, HEIR treatment typically necessary. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Glial fibrillary acidic protein (GFAP)
  • Specificity The specificity of this antibody has been confirmed by WB. Human, Rat, Mouse, Feline. Predicted to react with other mammals.
  • Target Host Species Human
  • Species Reactivity Cat, Human, Mouse, Other Mammals (Predicted), Rat
  • Antibody Host Chicken
  • Antibody Type Polyclonal
  • Antibody Isotype IgY
  • Conjugate Unconjugated
  • Immunogen Description Recombinant GFAP (expressed in E.coli) and native bovine GFAP
  • Purity Description IgY
  • Format Lyophilized IgY preparation, with sodium azide.
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Astrocyte; Glial fibrillary acidic protein; GFAP;
  • Uniprot Number Q28115
  • Uniprot Number/Name Q28115 (GFAP_BOVIN)
  • Scientific Background Glial fibrillary acidic protein (GFAP) is approx. 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Mixed cultures of neurons and glia stained with Chicken polyclonal antibody to Glial Fibrillary Acidic Protein C-1373-50 (red) and DNA (blue). Astrocytes stain strongly and specifically in a clearly filamentous fashion with this antibody.

  • Left: Analysis of GFAP expression (green) in mouse hippocampus section by Immunohistochemistry. Section was stained with chicken anti-GFAP antibody (1:5,000) and co-stained with rabbit antibody to FOX3/NeuN (R-3770-100, 1:5,000, red). Blue: DAPI nuclear stain. IHC Method: Following transcardial perfusion with 4% PFA, mouse brain was post fixed for 24 hours, cut into 45 uM sections, and free-floating sections were stained. The GFAP antibody stains a network of astroglial cells, while the Fox3/NeuN antibody stains the nuclei and proximal perikarya of neurons. Right: Western blot analysis of GFAP expression in whole rat (2) and mouse (3) brain lysates using chicken anti-GFAP antibody (1:5,000). The strong band at about 50 kDa corresponds to GFAP protein. Smaller proteolytic fragments and alternate transcripts of GFAP may also be detected on such blots.

    Citations & References

  • Specific References Stallings NR et al. (2023) Long-term normalization of calcineurin activity in model mice rescues Pin1 and attenuates Alzheimer’s phenotypes without blocking peripheral T cell IL-2 response Alzheimers Res Ther. 15(1):179. Application: Mouse, IHC (IF).

    Hascup KN et al. (2020) Riluzole attenuates glutamatergic tone and cognitive decline in AβPP/PS1 mice. J Neurochem. [Epub ahead of print] Application: Mouse, IHC(IF).
  • General References Brenner M. et al (2001) Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease. Nat Genet. 2001 Jan;27(1):117-20.