Alternative NamesS100B; Calcium binding protein B1;
Application(s)IHC-Frozen, IHC-Paraffin-embedded
Antibody HostMouse
Antibody TypeMonoclonal
SpecificityThe specificity of this antibody has been confirmed by IHC against the antigen. Human, rabbit and rat. May cross-react with horse and monkey S100-beta based on amino acid sequence similarities.
Species ReactivityBovine, Horse (Predicted), Human, Primate (Predicted), Rabbit, Rat
Application DetailsImmunohistochemistry (IHC). A concentration of 1.0-2.0 µg/mL is recommended to detect S100 beta subunit in paraffin embedded tissues. Antigen retrieval by heat is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetProtein S-100 beta
SpecificityThe specificity of this antibody has been confirmed by IHC against the antigen. Human, rabbit and rat. May cross-react with horse and monkey S100-beta based on amino acid sequence similarities.
Target Host SpeciesBovine
Species ReactivityBovine, Horse (Predicted), Human, Primate (Predicted), Rabbit, Rat
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG
Clone NameSH-B1
ConjugateUnconjugated
Immunogen DescriptionBovine brain S-100b
Purity DescriptionAscites
FormatLyophilized from mouse ascites fluid containing 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS buffer to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage Instructions12 months after purchase at -20°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesS100B; Calcium binding protein B1;
Scientific BackgroundProtein S100 beta belongs to the S100 family of proteins containing 2 EF-hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Among brain cells, S100 beta is often used as a marker for astrocytes. The 2 subunits of S100 protein are the products of separate genes. The beta subunit is present in all S100 positive cells and tumours whereas the alpha subunit is detectable only in specific tissues such as heart, skeletal muscle and brain. The S100 protein is a homodimer composed of two alpha subunits (S100a) or two beta subunits (S100b) or as a heterodimer with one alpha and beta subunit (S100ab or S100ao). This antibody reacts specifically with the beta subunit.