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Myelin basic protein, degraded (dMBP), Rabbit Polyclonal Antibody

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Catalog Number
R-107
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    Product Info

  • Product Name Myelin basic protein, degraded (dMBP), Rabbit Polyclonal Antibody
  • Product Description google
    Rabbit anti-Myelin basic protein, demyelinating (MBP) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
  • Alternative Names

    Degenerating/ degraded Myelin Basic Protein; dMBP
  • Application(s) IHC-Frozen Degraded
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity

    This antibody recognises degraded MBP in demyelinated nerve tissues. Immunohistochemical analysis of lesioned rat spinal cord indicates a high level of specificity for this antiserum. This antiserum reacts with human and rat MBP. This antibody is particularly useful in neurodegenerative studies.
  • Species Reactivity Guinea Pig, Human, Rat
  • Immunogen Description A synthetic peptide (YG SLPQKSQRSQ DENPVV, aa: 68-86) as part of guinea pig MBP protein conjugated to KLH
  • Conjugate Unconjugated
  • Purity Description Whole serum
  • Regulatory Status For research use only.

    Specifications

  • Product Description
    Rabbit anti-Myelin basic protein, demyelinating (MBP) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
  • Application(s) IHC-Frozen Degraded
  • Application Details

    IHC, frozen, 4% PFA; A dilution of 1:1000 to 1:4000 is recommended. Antibody immunostains for MBP in abnormally appearing oligodendrocyte processes and cell bodies in demyelinating areas. This antibody recognises only areas of myelin degeneration when tested in injured spinal cord and lesioned sciatic nerves. It also stains discrete white matter in the brain of multiple system atrophy. Biosensis recommends that the end user determines the optimal dilutions/concentrations.
  • Target Myelin basic protein (MBP), degraded.
  • Specificity

    This antibody recognises degraded MBP in demyelinated nerve tissues. Immunohistochemical analysis of lesioned rat spinal cord indicates a high level of specificity for this antiserum. This antiserum reacts with human and rat MBP. This antibody is particularly useful in neurodegenerative studies.
  • Target Host Species Guinea pig
  • Species Reactivity Guinea Pig, Human, Rat
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype Mixed
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide (YG SLPQKSQRSQ DENPVV, aa: 68-86) as part of guinea pig MBP protein conjugated to KLH
  • Purity Description Whole serum
  • Format Lyophilized
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names

    Degenerating/ degraded Myelin Basic Protein; dMBP
  • Uniprot Number P25188
  • Uniprot Number/Name P25188 (MBP_CAVPO)
  • Scientific Background Myelin is a membrane characteristic of the nervous tissue and functions as an insulator to increase the velocity of the stimuli being transmitted between a nerve cell body and its target. Myelin isolated from human and bovine nervous tissue is composed of approximately 80% lipid and 20% protein, and 30% of the protein fraction constitutes myelin basic protein (MBP). MBP is an 'intrinsically unstructured' protein with a high proportion (approximately 75%) of random coil, but postulated to have core elements of beta-sheet and alpha-helix. MBP is a major protein in CNS myelin and is expressed specifically in the nervous system. A detailed immunochemical examination of monoclonal and polyclonal antibody responses to MBP and its peptides has revealed the existence of as many as 27 antigenic determinants, many of them conformational. Topological mapping of the potential antigenic determinants onto a model of MBP secondary structure places these determinants within 11 separate regions of the molecule, including those portions that have been found to be encephalitogenic. The message for myelin basic protein is selectively translocated to the ends of the cell processes. Immunization with myelin-associated antigens including MBP significantly promotes recovery after spinal cord contusion injury in the rat model. FUNCTION: Is, with PLP, the most abundant protein component of the myelin membrane in the CNS. Has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane (By similarity). SUBUNIT: Homodimer (By similarity). SUBCELLULAR LOCATION: Myelin membrane; peripheral membrane protein; cytoplasmic side. Cytoplasmic side of myelin. TISSUE SPECIFICITY: Found in both the central and the peripheral nervous system.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Immunohistochemistry on human brain with brain trauma (car accident), using rabbit antibody to guinea pig degraded myelin basic protein (68-86): whole serum (R-107-100) at a dilution of 1:2000. Courtesy of Dr. Wei-Ping Gai, The Flinders University of South Australia.