Myeloperoxidase (MPO), Rabbit Polyclonal Antibody

Only %1 left
Catalog Number
R-133
Discontinued

    Product Info

  • Product Name Myeloperoxidase (MPO), Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-Myeloperoxidase (MPO) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
  • Alternative Names MPO
  • Application(s) IHC-Frozen, WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity This antiserum reacts with human myeloperoxidase. Human
  • Species Reactivity Human
  • Immunogen Description Myeloperoxidase isolated from human polymorphonuclear leucocytes
  • Conjugate Unconjugated
  • Purity Description IgG
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-Myeloperoxidase (MPO) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
  • Application(s) IHC-Frozen, WB
  • Application Details IHC, WB. A dilution of 1:500-1:1000 is recommended. This antiserum works superbly for staining of paraffin-embedded tissue sections fixed in formalin, frozen sections and cell cytospins. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Myeloperoxidase (MPO)
  • Specificity This antiserum reacts with human myeloperoxidase. Human
  • Target Host Species Human
  • Species Reactivity Human
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description Myeloperoxidase isolated from human polymorphonuclear leucocytes
  • Purity Description IgG
  • Format Lyophilized
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 250 µL sterile-filtered 1X PBS, pH 7.2-7.6. Centrifuge to remove any insoluble material.
  • Storage Instructions After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names MPO
  • Uniprot Number P05164
  • Uniprot Number/Name P05164 (PERM_HUMAN)
  • Scientific Background FUNCTION: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. MPO is an important marker for myeloid cells, from the promyelocyte stage and to the mature forms. CATALYTIC ACTIVITY: Donor + H2O2 = oxidized donor + 2 H2O. CATALYTIC ACTIVITY: Cl- + H2O2 = HOCl + 2 H2O. COFACTOR: Binds 1 calcium ion per heterodimer. COFACTOR: Binds 1 heme B (iron-protoporphyrin IX) group covalently per heterodimer. SUBUNIT: Tetramer of two light chains and two heavy chains. SUBCELLULAR LOCATION: Lysosome. ALTERNATIVE PRODUCTS: 3 named isoforms produced by alternative splicing. DISEASE: Defects in MPO are the cause of myeloperoxidase deficiency (MPD). MPD is an autosomal recessive defect that results in disseminated candidiasis. SIMILARITY: Belongs to the peroxidase family. XPO subfamily. Microglia and astrocytes are known to express MPO as well.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

    Citations & References