SpecificityThe specificity of this antibody has been confirmed by WB against the antigen. Human; rat; predicted to react with mouse due to sequence homology;
Species ReactivityHuman, Mouse (Predicted), Rat
Immunogen DescriptionA synthetic peptide (DKVYQMKSKPRGYCLIINNH) corresponding to a region (223-242 aa) from human Caspase-8.
Product DescriptionRabbit anti-Caspase-8 (CASP-8) Polyclonal Antibody (Unconjugated), suitable for WB.
Application(s)WB
Application DetailsWestern Blotting (WB). A concentration of 1.0 µg/mL is recommended for WB. Human Caspase-8 (Isoform 1 precursor) has a predicted length of 479 amino acids and MW of 55 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetCaspase-8 (CASP-8)
SpecificityThe specificity of this antibody has been confirmed by WB against the antigen. Human; rat; predicted to react with mouse due to sequence homology;
Target Host SpeciesHuman
Species ReactivityHuman, Mouse (Predicted), Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide (DKVYQMKSKPRGYCLIINNH) corresponding to a region (223-242 aa) from human Caspase-8.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundCaspase-8 is involved in the programmed cell death induced by Fas and various apoptotic stimuli. Many alternatively spliced transcript variants encoding different isoforms have been described. (Ref: Entrez Gene). Recent observations in microglia have implicated caspase 8 as a modulator of microglial activation in absence of cell death.