SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; predicted to react with mouse due to sequence homology;
Species ReactivityHuman, Mouse (Predicted), Rat
Immunogen DescriptionA synthetic peptide (EMARHLQEYQDLLNVK) corresponding to a region (341-356) from human Glial Fibrillary Acidic Protein. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
ConjugateUnconjugated
Purity DescriptionAffinity purified on antigen column
Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0 µg/mL is recommended for WB. Human GFAP has a predicted length of 432 residues and MW of 50 kDa. A concentration of 2.0 µg/mL is recommended to detect GFAP in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetGlial Fibrillary Acidic Protein (GFAP)
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; predicted to react with mouse due to sequence homology;
Target Host SpeciesHuman
Species ReactivityHuman, Mouse (Predicted), Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide (EMARHLQEYQDLLNVK) corresponding to a region (341-356) from human Glial Fibrillary Acidic Protein. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
Purity DescriptionAffinity purified on antigen column
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesAstrocyte; Glial fibrillary acidic protein; GFAP;
Scientific BackgroundGlial Fibrillary Acidic Protein (GFAP) is a 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas. At least 3 isoforms are produced from alternate splicing. These isoforms differ in the C-terminal region which is encoded by alternative exons.
General ReferencesReeves S.A, et al. Proc. Natl. Acad. Sci. U.S.A. 86:5178-5182(1989). Brenner M, et al. Brain Res. Mol. Brain Res. 7:277-286(1990). Isaacs A, et al. Genomics 51:152-154(1998). Ota T, et al. Nat. Genet. 36:40-45(2004). Nielsen A.L, et al. J. Biol. Chem. 277:29983-29991(2002). Singh R, et al. Genomics 82:185-193(2003). Brenner M, et al. Nat. Genet. 27:117-120(2001). Brockmann K, et al. Eur. Neurol. 50:100-105(2003). Stumpf E, et al. Arch. Neurol. 60:1307-1312(2003). Sawaishi Y, et al. Neurology 58:1541-1543(2002). Aoki Y, et al. Neurosci. Lett. 312:71-74(2001).