Myeloperoxidase (MPO), Rabbit Polyclonal Antibody

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Catalog Number
R-1073

    Product Info

  • Product Name Myeloperoxidase (MPO), Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-Myeloperoxidase (MPO) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
  • Alternative Names MPO; EC 1.11.1.7; Myeloperoxidase;
  • Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; predicted to react with mouse
  • Species Reactivity Human, Mouse (Predicted), Rat
  • Immunogen Description A synthetic peptide (KNNIFMSNSYPRDFV) corresponding to a region (714-728) at the C-terminus of human Myeloperoxidase (MPO).
  • Conjugate Unconjugated
  • Purity Description Affinity purified on antigen column
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-Myeloperoxidase (MPO) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
  • Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
  • Application Details Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human MPO (isoform H17) has a predicted length of 745 residues and MW of 84 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect the protein in formalin/paraffin embedded tissues. Heat mediated antigen retrieval in 10 mM citrate buffer, pH 6.0 for 20 minutes is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Myeloperoxidase (MPO)
  • Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; predicted to react with mouse
  • Target Host Species Human
  • Species Reactivity Human, Mouse (Predicted), Rat
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide (KNNIFMSNSYPRDFV) corresponding to a region (714-728) at the C-terminus of human Myeloperoxidase (MPO).
  • Purity Description Affinity purified on antigen column
  • Format Lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
  • Storage Instructions At least 12 months after purchase at -20°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names MPO; EC 1.11.1.7; Myeloperoxidase;
  • Uniprot Number P05164
  • Uniprot Number/Name P05164 (PERM_HUMAN)
  • Scientific Background FUNCTION: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. MPO is an important marker for myeloid cells, from the promyelocyte stage and to the mature forms. CATALYTIC ACTIVITY: Donor + H2O2 = oxidized donor + 2 H2O. CATALYTIC ACTIVITY: Cl- + H2O2 = HOCl + 2 H2O. COFACTOR: Binds 1 calcium ion per heterodimer. COFACTOR: Binds 1 heme B (iron-protoporphyrin IX) group covalently per heterodimer. SUBUNIT: Tetramer of two light chains and two heavy chains. SUBCELLULAR LOCATION: Lysosome. ALTERNATIVE PRODUCTS: At least 3 named isoforms produced by alternative splicing. DISEASE: Defects in MPO are the cause of myeloperoxidase deficiency (MPD). MPD is an autosomal recessive defect that results in disseminated candidiasis. SIMILARITY: Belongs to the peroxidase family. XPO subfamily. Microglia and astrocytes are known to express MPO as well.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Immunohistochemical staining using rabbit polyclonal antibody to human Myeloperoxidase in paraffin-embedded sections of human ovary at 0.5-1 µg/mL. Secondary biotinylated goat anti-rabbit IgG antibody at 10 µg/mL.