SpecificityThe specificity of this antibody has been tested by WB on mouse tissue. Mouse. Other species not yet tested.
Species ReactivityMouse
Immunogen DescriptionA synthetic peptide corresponding to a sequence at the N-terminus of mouse HGF (33-64) (QKKRRNTLHEFKKSAKTTLTKEDPLLKIKTKK). Different from therelated human sequence by five amino acids, and from the related rat sequence by one amino acid.
Application DetailsWestern Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetHepatocyte Growth Factor (HGF)
SpecificityThe specificity of this antibody has been tested by WB on mouse tissue. Mouse. Other species not yet tested.
Target Host SpeciesMouse
Species ReactivityMouse
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide corresponding to a sequence at the N-terminus of mouse HGF (33-64) (QKKRRNTLHEFKKSAKTTLTKEDPLLKIKTKK). Different from therelated human sequence by five amino acids, and from the related rat sequence by one amino acid.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsStore lyophilized product at -20°C. After reconstitution keep aliquots at -20°C for a higher stability. Working solution can be kept at 2-8°C for up to 4 weeks. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundHepatocyte growth factor (HGF) is a member of the plasminogen related growth factor family. The HGF protein is a dimer formed from an alpha and beta subunit linked by a disulfide bond. Both subunits originate from the endoproteolytic cleavage of a common precursor which is biologically inactive. HGF is expressed in Kupffer cells and sinusoidal endothelial cells in the liver and pancreas.