Epidermal growth factor receptor (EGFR), Rabbit Polyclonal Antibody

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Catalog Number
R-1826
Discontinued

    Product Info

  • Product Name Epidermal growth factor receptor (EGFR), Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-Epidermal growth factor receptor (EGFR) Polyclonal Antibody (Unconjugated), suitable for WB.
  • Alternative Names Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; EGFR; ERBB;
  • Application(s) WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity This antibody is specific for EGFR as demonstrated by western blotting.
  • Species Reactivity Human, Rat
  • Immunogen Description E.coli-derived human EGFR recombinant protein (Position: L25-K346). Human EGFR shares 89% amino acid (aa) sequence identity with mouse EGFR.
  • Conjugate Unconjugated
  • Purity Description Affinity purified
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-Epidermal growth factor receptor (EGFR) Polyclonal Antibody (Unconjugated), suitable for WB.
  • Application(s) WB
  • Application Details Western Blot (0.1-0.5 µg/mL): tested on human and rat cell lines. Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Epidermal growth factor receptor (EGFR)
  • Specificity This antibody is specific for EGFR as demonstrated by western blotting.
  • Target Host Species Human
  • Species Reactivity Human, Rat
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description E.coli-derived human EGFR recombinant protein (Position: L25-K346). Human EGFR shares 89% amino acid (aa) sequence identity with mouse EGFR.
  • Purity Description Affinity purified
  • Format Lyophilized
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 uL sterile-filtered, ultrapure water to prepare 1 mg/mL solution. Centrifuge to remove any insoluble material.
  • Storage Instructions Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; EGFR; ERBB;
  • Uniprot Number P00533
  • Uniprot Number/Name P00533 (EGFR_HUMAN)
  • Scientific Background Epidermal Growth Factor (EGF) Receptor binds to EGF and other members of the EGF family such as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. At least 4 isoforms are produced by alternative splicing (reference SWISSPROT). The EGF receptor is a single-pass type I membrane protein containing an extracellular receptor domain, a transmembrane region and an intracellular domain with tyrosine kinase function
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Western Blot analysis of EGFR expression in HELA (1), A549 (2) and A431 (3) whole cell lysates (40 µg/lane). The observed molecular weight of ~200 kDa corresponds to glycosylated EGFR.

    Citations & References