Application DetailsWestern Blotting (WB). A concentration of 1.0 µg/mL is recommended for WB. Rat Interleukin 6 (precursor) has a predicted length of 211 amino acids and MW of 24 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetInterleukin 6 (IL-6)
SpecificityThe specificity of this antibody has been confirmed by WB against the antigen. Rat; mouse;
Target Host SpeciesRat
Species ReactivityMouse, Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide corresponding to a region (195-211 aa) from rat Interleukin 6 (IL-6).
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundIL-6 is a cytokine with many biological functions. Some of its roles include the final differentiation of B-cells into Ig-secreting cells, induction of meloma and plasmacytoma growth, induction of nerve cell differentiation, and in hepatocytes, induction of acute phase reactants. IL-6 is secreted and is N- and O-glycosylated post translationally.