Product NameAdaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), Rabbit Polyclonal Antibody
Product DescriptiongoogleRabbit anti-Adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Alternative NamesDCC-interacting protein 13-beta; Dip13-beta; Adapter protein containing PH domain; PTB domain and leucine zipper motif 2; APPL2; DIP13B;
Application(s)ICC, WB
Antibody HostRabbit
Antibody TypePolyclonal
SpecificityThis antiserum is known to recognise both mouse and human APPL2.
Species ReactivityHuman, Mouse
Immunogen DescriptionMouse APPL2 protein (amino acids: 619-662) conjugated to GST.
Product DescriptionRabbit anti-Adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Application(s)ICC, WB
Application DetailsWestern Blotting (WB) and Immunoprecipitation (IP). A dilution between 1:2500 and 1:5000 is recommended for WB. A concentration of 3 µL/reaction is recommended for IP. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetAdaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2)
SpecificityThis antiserum is known to recognise both mouse and human APPL2.
Target Host SpeciesMouse
Species ReactivityHuman, Mouse
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeMixed
ConjugateUnconjugated
Immunogen DescriptionMouse APPL2 protein (amino acids: 619-662) conjugated to GST.
Purity DescriptionWhole serum
FormatLiquid. 15% glycerol
Storage InstructionsAliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesDCC-interacting protein 13-beta; Dip13-beta; Adapter protein containing PH domain; PTB domain and leucine zipper motif 2; APPL2; DIP13B;
Scientific BackgroundAPPL2 is an isoform of APPL1 with 54% identity in their protein sequences. APPL2 has a role in cell proliferation and embryonic development. Recently, it was shown that APPL2 regulates FSH signaling and acts as a negative regulator in adiponectin signaling.
Western blot analysis with Rabbit polyclonal antibody to mouse APPL2, catalog number R-1332-100. Tissue homogenates of mouse brain (B), liver (Li), muscle (M), fat (F), heart (H), kidney (K), spleen (S) and pancreas (P) were used and 30 µg of protein was loaded in each lane. Image courtesy of Wang C. et al (2009) J Biol Chem.