Product NameNon-specific Control IgG, Mouse Monoclonal Antibody (Saporin)
Product DescriptiongoogleX63-saporin is an antibody conjugate comprising of the non-specific monoclonal IgG1 antibody X63, chemically conjugated via a reducible disulfide bridge to the ribosome-inactivating protein saporin, purified from saponaria officinalis. Antibody clone X63 has no known binding ability, and thus can be used as negative control antibody. In combination with saporin, X63-saporin is a useful negative control for targeting IgG1-saporin conjugates such as MC192-saporin.
Application(s)Negative Control
Antibody HostMouse
Antibody TypeMonoclonal
SpecificityNo staining has ever been identified with this immunoglobulin demonstrating its non-specific value as a control.
Species ReactivityNon-Reactive (Negative Control)
Immunogen DescriptionUnknown, naturally isolated IgG that has no known binding target in mammals
ConjugateSaporin
Purity DescriptionConjugate was purified by ion-exchange chromatography. Purity > 90% by non-reducing SDS-PAGE
Product DescriptionX63-saporin is an antibody conjugate comprising of the non-specific monoclonal IgG1 antibody X63, chemically conjugated via a reducible disulfide bridge to the ribosome-inactivating protein saporin, purified from saponaria officinalis. Antibody clone X63 has no known binding ability, and thus can be used as negative control antibody. In combination with saporin, X63-saporin is a useful negative control for targeting IgG1-saporin conjugates such as MC192-saporin.
Application DetailsNegative control for targeting IgG1-saporin conjugates., for instance MC192-saporin. X63-saporin should be used at a concentration equal to that of the test antibody-saporin conjugate.
TargetIsotype control
SpecificityNo staining has ever been identified with this immunoglobulin demonstrating its non-specific value as a control.
Target Host SpeciesSpecies Independent
Species ReactivityNon-Reactive (Negative Control)
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG1, kappa
Clone NameX63
ConjugateSaporin
Immunogen DescriptionUnknown, naturally isolated IgG that has no known binding target in mammals
Biological ActivityNone. Routinely tested for absence of killing of rat C6 cells in vitro. Note that the primary use of X63-saporin is for in vivo applications.
Purity DescriptionConjugate was purified by ion-exchange chromatography. Purity > 90% by non-reducing SDS-PAGE
FormatLyophilized from a 1 mg/mL solution containing PBS pH 7.2-7.6 without preservative.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in sterile-filtered, ultrapure water to achieve a conjugate concentration of 1 mg/mL. Centrifuge to remove any insoluble material. Final buffer contains no preservatives.
Storage InstructionsLyophilized product is shipped at ambient room temperature. Upon receipt, pulse-centrifuge the vial to collect solid that may be entrapped in the lid. After reconstitution, immediately prepare aliquots and keep the undiluted stock at -80°C for long-term storage. Avoid repeated thaw-freezing. For short-term storage, keep at 2-8°C for up to 2 weeks. it is recommended to handle this product under sterile conditions.
Hazardous StatementOnly qualified personnel should handle this product. X63 antibody has no known target receptor, however, standard laboratory protective measures should be followed, including wearing lab coat, gloves and safety glasses. Avoid contact with open wounds and practice good laboratory technique to avoid auto-exposure/auto-injection! Avoid contact of conjugate with reducing agents (eg., DTT, beta-mercaptoethanol) as this will destroy the antibody-saporin conjugate.