Application DetailsWestern Blotting (WB). A concentration of 2.0-4.0 µg/mL is recommended for WB. Human alpha adaptin (isoform #1) has a predicted length of 977 residues and MW of 108 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetAP-2 complex subunit alpha-1
SpecificityThe specificity of this antibody has been confirmed by WB against the antigen. Human; rat;
Target Host SpeciesBovine
Species ReactivityBovine, Human, Rat
Immunogen DescriptionAssembly polypeptide 2 (AP2) adaptor polypeptides from bovine brain
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundAssembly Polypeptides (AP), also known as adaptors, co-assemble with clathrin during the formation of clathrin coated membranes. AP2 adaptors are associated with the endocytic clathrin coated vesicles at the plasma membrane. The AP2 adaptor is composed of four subunits which includes alpha- and beta-adaptin. This antibody reacts specifically with the alpha-adaptin subunit of the AP2 adaptor.