Application DetailsThis antibody is recommended for WB and direct ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetDynactin subunit 1
SpecificityThe specificity of this antibody has been confirmed by WB and ELISA against the recombinant protein. No other species have been tested.
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG
Clone Name1E12
ConjugateUnconjugated
Immunogen DescriptionPartial sequence recombinant protein of human dynactin subunit 1 (amino acids 1082-1270) with GST tag
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.2
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative Names150 kDa dynein-associated polypeptide; DP-150; DAP-150; p150-glued; p135; DCTN1
Dynactin subunit 1 (DCTN1) is part of a large macromolecular complex of at least 10 components. It is required for cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles. Dynactin subunit 1 binds directly to microtubules and to cytoplasmic dynein. It is expressed in the brain and localises to the cytoplasm and the cytoskeleton. It belongs to the dynactin 150 kDa subunit family and contains one CAP-Gly domain. Abnormalities in DCTN1 cause progressive lower motor neuron disease (PLMND).
Western blot detection of GST tagged recombinant protein using mouse monoclonal antibody to human dynactin subunit 1, catalogue number M-822-100. Note, the GST tag alone has a molecular weight of 26 kDa.