Product DescriptionMouse anti-Ena/VASP-like protein Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Application DetailsThis antibody is recommended for WB, and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificitySpecificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Target Host SpeciesHuman
Immunogen DescriptionPartial recombinant human Ena/VASP-like protein (309-419) with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.2
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesEna/vasodilator-stimulated phosphoprotein-like; EVL; RNB6
Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. Ena/VASP-like protein enhances actin nucleation ad polymerisation. It is required to transform actin polymerisation into active movement for the propulsive force of Listeria monocytogenes. It exists as a homotetramerwhich localises to the cytoplasm, cytoskeleton and cell projection. There are two name isoforms produced by alternative splicing.
Western blot detection of Ena/VASP-like protein expression in Transfected 293T cell lysate. This antibody detects a band of molecular weight 45 kDa in Ena/VASP-like protein transfected lysate (lane 1) and is negative against non-transfected 293T lysate.
Sandwich ELISA using anti-Ena/VASP-like protein monoclonal antibody as a capture antibody. The detection limit for GST tagged recombinant antigen is 0.03 ng/mL.