Ena/VASP-like protein, Mouse Monoclonal Antibody

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Catalog Number
M-898

    Product Info

  • Product Name Ena/VASP-like protein, Mouse Monoclonal Antibody
  • Product Description google Mouse anti-Ena/VASP-like protein Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
  • Alternative Names Ena/vasodilator-stimulated phosphoprotein-like; EVL; RNB6
  • Application(s) ELISA, WB
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Specificity Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
  • Species Reactivity Human
  • Immunogen Description Partial recombinant human Ena/VASP-like protein (309-419) with a GST tag.
  • Conjugate Unconjugated
  • Purity Description Protein G purified immunoglobulin
  • Regulatory Status For research use only.

    Specifications

  • Product Description Mouse anti-Ena/VASP-like protein Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
  • Application(s) ELISA, WB
  • Application Details This antibody is recommended for WB, and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Ena/VASP-like protein
  • Specificity Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
  • Target Host Species Human
  • Species Reactivity Human
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Antibody Isotype IgG
  • Clone Name 5G1
  • Conjugate Unconjugated
  • Immunogen Description Partial recombinant human Ena/VASP-like protein (309-419) with a GST tag.
  • Purity Description Protein G purified immunoglobulin
  • Format Lyophilized from PBS pH 7.2
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions After reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Ena/vasodilator-stimulated phosphoprotein-like; EVL; RNB6
  • Uniprot Number Q9UI08
  • Uniprot Number/Name Q9UI08 (EVL_HUMAN)
  • Scientific Background
    Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. Ena/VASP-like protein enhances actin nucleation ad polymerisation. It is required to transform actin polymerisation into active movement for the propulsive force of Listeria monocytogenes. It exists as a homotetramerwhich localises to the cytoplasm, cytoskeleton and cell projection. There are two name isoforms produced by alternative splicing.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Western blot detection of Ena/VASP-like protein expression in Transfected 293T cell lysate. This antibody detects a band of molecular weight 45 kDa in Ena/VASP-like protein transfected lysate (lane 1) and is negative against non-transfected 293T lysate.

  • Sandwich ELISA using anti-Ena/VASP-like protein monoclonal antibody as a capture antibody. The detection limit for GST tagged recombinant antigen is 0.03 ng/mL.

    Citations & References