Application DetailsThis antibody is recommended for WB, IHC and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetFilamin-A
SpecificitySpecificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG
Clone Name4E10-1B2
ConjugateUnconjugated
Immunogen DescriptionPartial recombinant protein of human Filamin A (aa 1809 to 2647) with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.2
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesAlpha-filamin; Filamin-1; Endothelial actin-binding protein; Actin-binding protein 280; ABP-280; Non-muscle filamin; FLNA; FLN; FLN1
Filamin A, is a 280- kD protein that promotes orthogonal branching of actin filaments cortical cytoplasm. It anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. Remodeling of the cytoskeleton is central to the modulation of cell shape and migration.
Western blot detection of Filamin A in human promyelocytic leukemia cell lysate using mouse monoclonal to human Filamin A, catalogue number M-835-100.
Immunohistochemical detection of Filamin A in formalin-fixed paraffin-embedded human lymphoma tissue using mouse monoclonal to human Filamin A, catalogue number M-835-100.