Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. A concentration of 0.5-1.0 µg/mL is recommended to detect GLUT4 in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Glucose transporter 4 (GLUT-4)
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Rat; predicted to react with human and mouse due to sequence homology;
Target Host SpeciesHuman
Species ReactivityHuman (Predicted), Mouse (Predicted), Rat
Immunogen DescriptionA synthetic peptide corresponding to the region (491-509 aa) from the C-terminus of human GLUT4.
Purity DescriptionAffinity purified on antigen column
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 µg/µL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundGlucose transporter 4 (GLUT4) is a multi-pass membrane protein. It is a member of the family of glucose transporters that facilitate glucose uptake by cells. GLUT4 is sequestered within cells not stimulated by insulin. After insulin stimulation, it is translocated to the cell surface where it transports glucose across the cell membrane. GLUT4 is found in skeletal and heart muscles and in adipose tissue. It is highly conserved between species.