Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0-2.0 µg/mL is recommended for WB. Human GLUT1 has a predicted length of 492 residues and MW of 55 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect GLUT1 in formalin fixed and paraffin embedded tissues. Antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetGlucose transporter 1 (GLUT-1)
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat;
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Immunogen DescriptionA synthetic peptide (FYNQTWVHRYGESI) corresponding to a region (43-56) from the N-terminus of human Glucose transporter 1 (GLUT1).
Purity DescriptionAffinity purified on antigen column
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 µg/µL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundGlucose transporter 1 (GLUT1) is a multi-pass membrane protein. It is a member of the family of glucose transporters that facilitate glucose uptake by cells. GLUT1 is a major transporter at the mammalian blood-brain barrier.