Alternative NamesCatenin beta-1; Beta-catenin; CTNNB1; CTNNB; _-Catenin;
Application(s)ICC, IHC-Frozen, WB
Antibody HostSheep
Antibody TypePolyclonal
SpecificityThe specificity of this antibody has been confirmed by WB against the antigen. Human; mouse; rat;
Species ReactivityHuman, Mouse, Rat
Immunogen DescriptionA synthetic peptide (C-QSYLDSGIHSGATTTAPSL) corresponding to the amino acid region 28-46 of human Catenin beta coupled to carrier protein KLH. This antibody was designed to detect the active form of Catenin beta . The antibody exhibits a strong preference for dephosphorylated beta catenin protein by western blot but the antibody may show cross reactivity with phosphorylated catenin beta under certain conditions.
Application DetailsWestern Blotting (WB) and Immunocytochemistry. A concentration of 2.0 µg/mL is recommended for WB. Human Catenin beta (isoform 1) has a predicted length of 781 residues and MW of 86 kDa. It has been observed as a 92 kDa band in SDS-reducing gels. A concentration of 4.0 µg/mL is recommended for Immunocytochemistry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetCatenin beta
SpecificityThe specificity of this antibody has been confirmed by WB against the antigen. Human; mouse; rat;
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Antibody HostSheep
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide (C-QSYLDSGIHSGATTTAPSL) corresponding to the amino acid region 28-46 of human Catenin beta coupled to carrier protein KLH. This antibody was designed to detect the active form of Catenin beta . The antibody exhibits a strong preference for dephosphorylated beta catenin protein by western blot but the antibody may show cross reactivity with phosphorylated catenin beta under certain conditions.
Purity DescriptionAffinity purified
FormatLyophilized from 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, and 0.05 % sodium azide.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesCatenin beta-1; Beta-catenin; CTNNB1; CTNNB; _-Catenin;
Scientific BackgroundCatenin beta is an adherens junction protein and has a role in the regulation of cell adhesion and in signal transduction through the Wnt pathway. At least 2 isoforms are produced by alternative splicing.
Western blot analysis of Catenin beta (S-1233-100) in extracts from HepG2 cells: untreated (Lane 1) and treated with GSK3 inhibitor (Lane 2).
Staining at cell junctions of active Catenin beta in INS-E cells in culture using Sheep polyclonal antibody to dephosphorylated Catenin beta S-1233-100.