Catenin beta, Sheep Polyclonal Antibody

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Catalog Number
S-1233

    Product Info

  • Product Name Catenin beta, Sheep Polyclonal Antibody
  • Product Description google Sheep anti-Catenin beta Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
  • Alternative Names Catenin beta-1; Beta-catenin; CTNNB1; CTNNB; _-Catenin;
  • Application(s) ICC, IHC-Frozen, WB
  • Antibody Host Sheep
  • Antibody Type Polyclonal
  • Specificity The specificity of this antibody has been confirmed by WB against the antigen. Human; mouse; rat;
  • Species Reactivity Human, Mouse, Rat
  • Immunogen Description A synthetic peptide (C-QSYLDSGIHSGATTTAPSL) corresponding to the amino acid region 28-46 of human Catenin beta coupled to carrier protein KLH. This antibody was designed to detect the active form of Catenin beta . The antibody exhibits a strong preference for dephosphorylated beta catenin protein by western blot but the antibody may show cross reactivity with phosphorylated catenin beta under certain conditions.
  • Conjugate Unconjugated
  • Purity Description Affinity purified
  • Regulatory Status For research use only.

    Specifications

  • Product Description Sheep anti-Catenin beta Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
  • Application(s) ICC, IHC-Frozen, WB
  • Application Details Western Blotting (WB) and Immunocytochemistry. A concentration of 2.0 µg/mL is recommended for WB. Human Catenin beta (isoform 1) has a predicted length of 781 residues and MW of 86 kDa. It has been observed as a 92 kDa band in SDS-reducing gels. A concentration of 4.0 µg/mL is recommended for Immunocytochemistry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Catenin beta
  • Specificity The specificity of this antibody has been confirmed by WB against the antigen. Human; mouse; rat;
  • Target Host Species Human
  • Species Reactivity Human, Mouse, Rat
  • Antibody Host Sheep
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide (C-QSYLDSGIHSGATTTAPSL) corresponding to the amino acid region 28-46 of human Catenin beta coupled to carrier protein KLH. This antibody was designed to detect the active form of Catenin beta . The antibody exhibits a strong preference for dephosphorylated beta catenin protein by western blot but the antibody may show cross reactivity with phosphorylated catenin beta under certain conditions.
  • Purity Description Affinity purified
  • Format Lyophilized from 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, and 0.05 % sodium azide.
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Catenin beta-1; Beta-catenin; CTNNB1; CTNNB; _-Catenin;
  • Uniprot Number P35222
  • Uniprot Number/Name P35222 (CTNB1_HUMAN)
  • Scientific Background Catenin beta is an adherens junction protein and has a role in the regulation of cell adhesion and in signal transduction through the Wnt pathway. At least 2 isoforms are produced by alternative splicing.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Western blot analysis of Catenin beta (S-1233-100) in extracts from HepG2 cells: untreated (Lane 1) and treated with GSK3 inhibitor (Lane 2).

  • Staining at cell junctions of active Catenin beta in INS-E cells in culture using Sheep polyclonal antibody to dephosphorylated Catenin beta S-1233-100.

    Citations & References