SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; predicted to react with mouse due to sequence homology;
Species ReactivityHuman, Mouse (Predicted), Rat
Immunogen DescriptionA synthetic peptide (PGNPKWERTNLTY) corresponding to a region (103-115) from human Matrix metalloproteinase-8. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
ConjugateUnconjugated
Purity DescriptionAffinity purified on antigen column
Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.75 µg/mL is recommended for WB. Human MMP8 (precursor) has a predicted length of 467 residues and MW of 53 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Matrix metalloproteinase-8 (MMP-8)
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; predicted to react with mouse due to sequence homology;
Target Host SpeciesHuman
Species ReactivityHuman, Mouse (Predicted), Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide (PGNPKWERTNLTY) corresponding to a region (103-115) from human Matrix metalloproteinase-8. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
Purity DescriptionAffinity purified on antigen column
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundThe matrix metalloproteinases (MMPs) are a large family of zinc endopeptidases. All MMPs are synthesized as inactive proenzymes. The activation of these proenzymes is a critical step that leads to degradation of extracellular matrix components such as fibronectin and collagen type III. MMP8 is known to degrade fibrillar type I, II, and III collagens.