Internexin alpha (Alpha-Inx), Rabbit Polyclonal Antibody

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Catalog Number
R-1379

    Product Info

  • Product Name Internexin alpha (Alpha-Inx), Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-Internexin alpha (Alpha-Inx) Polyclonal Antibody (Unconjugated), suitable for WB, ICC, IHC-Frozen.
  • Alternative Names 67 kDa neurofilament protein; NF-66; Neurofilament-66; Alpha-internexin; Alpha-Inx; Neurofilament 5; INA; NEF5;
  • Application(s) ICC, IHC-Frozen, WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity The specificity of this antibody has been confirmed by WB. This antibody is specific for the 64-66 kDa alpha-internexin protein. Molecular weight will depend on species. Hu, Rat, Ms, Fel, and other mammals
  • Species Reactivity Cat, Human, Mouse, Other Mammals, Rat
  • Immunogen Description Recombinant alpha-internexin expressed and purified from E. coli
  • Conjugate Unconjugated
  • Purity Description Whole serum
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-Internexin alpha (Alpha-Inx) Polyclonal Antibody (Unconjugated), suitable for WB, ICC, IHC-Frozen.
  • Application(s) ICC, IHC-Frozen, WB
  • Application Details Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:10,000 - 1:20,000 is recommended for WB. A dilution of 1:500-1,000 is recommended for ICC and IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Internexin alpha (Alpha-Inx)
  • Specificity The specificity of this antibody has been confirmed by WB. This antibody is specific for the 64-66 kDa alpha-internexin protein. Molecular weight will depend on species. Hu, Rat, Ms, Fel, and other mammals
  • Target Host Species Rat
  • Species Reactivity Cat, Human, Mouse, Other Mammals, Rat
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype Mixed
  • Conjugate Unconjugated
  • Immunogen Description Recombinant alpha-internexin expressed and purified from E. coli
  • Purity Description Whole serum
  • Format Lyophilized with sodium azide.
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names 67 kDa neurofilament protein; NF-66; Neurofilament-66; Alpha-internexin; Alpha-Inx; Neurofilament 5; INA; NEF5;
  • Uniprot Number P23565
  • Uniprot Number/Name P23565 (AINX_RAT)
  • Scientific Background Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, alpha-internexin, nestin and in some cases vimentin. Alpha-internexin is a ~66 kDa Class IV intermediate filament subunit expressed in large amounts early in neuronal development, but is downregulated in many neurons as development procedes. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of alpha-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Mixed neuron-glial cultures stained with Rabbit polyclonal antibody to Internexin alpha R-1379-50 (red) and Chicken polyclonal antibody to peripherin C-1399-50 (green). The alpha internexin antibody stains numerous axonal and dendritic profiles in these cultures, while Peripherin antibody binds to only a subset of neurons.

  • Left: Detection of alpha-internexin expression (green) in rat cerebellum by Immunohistochemistry. The rabbit antibody to alpha-internexin was used at 1:2,000 dilution, and section was co-stained with chicken antibody to GFAP (C-1373-50, red, 1:5,000). Blue: DAPI nuclear stain. IHC Method: Following transcardial perfusion with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut to 45 um, and free-floating sections were stained. The alpha-internexin antibody selectively stains axons and dendrites of neuronal cells, in particular Purkinje cells and parallel fibers the axons of granule cells. The GFAP antibody labels network of glial cells, such as astrocytes in the granule cell layer and white matter and Bergmann glia in the molecular layer. Right: Western blot analysis of whole tissue lysates using rabbit antibody to alpha-internexin (green, 1:10,000). [1] protein standard, [2] mouse spinal cord, [3] rat spinal cord, [4] bovine spinal cord. Major bands in the 64-66 kDa range correspond to alpha-internexin. The alpha-internexin protein from different species is known to vary slightly in molecular weight as determined by SDS-PAGE.

    Citations & References