Application DetailsELISA. Suggested dilution at 1:500 to 1:2,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
TargetAppetite-regulating hormone (Grehlin)
SpecificityAntibody to Ghrelin (17-28): Affinity purified
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Antibody HostChicken
Antibody TypePolyclonal
Antibody IsotypeIgY
ConjugateUnconjugated
Immunogen DescriptionSynthetic peptide from human Ghrelin, C-terminal, (17-28 aa)
SequenceESKKPPAKLQPR
Purity DescriptionAffinity purified
FormatLiquid. PBS with 0.02% Sodium Azide
Storage InstructionsShort term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundGhrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR) and upon binding to the receptor it induces the release of growth hormone from the pituitary. This ligand has an appetite-stimulating effect and is involved in growth regulation (Ref: SWISSPROT).
Analysis of Ghrelin in Sandwich ELISA. Coating antibody was Chicken polyclonal antibody to Ghrelin (C-1519-200) and the detection antibody was Chicken polyclonal antibody to active Ghrelin (C-1525-200).