Melanocyte-stimulating hormone, gamma (Gamma-MSH), Guinea pig Polyclonal Antibody

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Catalog Number
GP-031

    Product Info

  • Product Name Melanocyte-stimulating hormone, gamma (Gamma-MSH), Guinea pig Polyclonal Antibody
  • Product Description google
    Guinea Pig anti-Melanocyte-stimulating hormone, gamma (Gamma-MSH) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
  • Alternative Names Gamma-melanocyte stimulating hormone; Melanotropin gamma; Corticotropin-lipotropin [Precursor]; Pro-opiomelanocortin; POMC
  • Application(s) IHC-Frozen
  • Antibody Host Guinea Pig
  • Antibody Type Polyclonal
  • Specificity Human. Specificity was demonstrated by immunohistochemistry. This antiserum is known to react with sheep gamma MSH. Other species have not yet been tested.
  • Species Reactivity Human, Sheep
  • Immunogen Description A synthetic peptide (YVMGHFRWDRFG) corresponding to the amino acids 77-88 from human Proopiomelanocortin (POMC). POMC is the precursor of the melanocortin peptides alpha, beta and gamma. The synthetic peptide was conjugated to carrier protein to enhance the immunological response.
  • Conjugate Unconjugated
  • Purity Description Whole serum
  • Regulatory Status For research use only.

    Specifications

  • Product Description
    Guinea Pig anti-Melanocyte-stimulating hormone, gamma (Gamma-MSH) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
  • Application(s) IHC-Frozen
  • Application Details IHC. A concentration of 1 in 2000 is recommended for IHC. This antibody shows excellent staining on sheep and human tissue. Rodent tissues demonstrate high background staining. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Melanocyte-stimulating hormone, gamma (Gamma-MSH)
  • Specificity Human. Specificity was demonstrated by immunohistochemistry. This antiserum is known to react with sheep gamma MSH. Other species have not yet been tested.
  • Target Host Species Human
  • Species Reactivity Human, Sheep
  • Antibody Host Guinea Pig
  • Antibody Type Polyclonal
  • Antibody Isotype Mixed
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide (YVMGHFRWDRFG) corresponding to the amino acids 77-88 from human Proopiomelanocortin (POMC). POMC is the precursor of the melanocortin peptides alpha, beta and gamma. The synthetic peptide was conjugated to carrier protein to enhance the immunological response.
  • Purity Description Whole serum
  • Format Lyophilized
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions Store lyophilized antibody at 2-8ºC or lower. After reconstitution, divide into aliquots and store at -20°C, or lower, for optimal long term stability. It is recommended that a reconstituted aliquot is stored at 2-8°C for no longer than 2 weeks. Allocation of an appropriate anti-bacterial agent can increase shelf life by several weeks. Glycerol (1:1) can be added to neat serum for additional stability if intended use does not prevent this.
  • Batch Number Please see item label.
  • Expiration Date

    12 months after date of receipt (lyophilized, unopened vial).

  • Alternative Names Gamma-melanocyte stimulating hormone; Melanotropin gamma; Corticotropin-lipotropin [Precursor]; Pro-opiomelanocortin; POMC
  • Uniprot Number P01189
  • Uniprot Number/Name P01189 (COLI_HUMAN)
  • Scientific Background
    Proopiomelanocortin (POMC) is the precursor of the melanocortin peptides alpha, beta and gamma. POMC neurons in the arcuate nucleus of the hypothalamus function as key central regulators of food intake and homeostatic control of energy balance in both rodents and non-human primates.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • The cryostat section of the sheep hypothalamus was incubated in guinea pig polyclonal antibodies to the gamma melanocyte-stimulating hormone at the dilution of 1:1000 overnight followed by incubation with FITC- conjugated secondary antibodies. Cell bodies and nerve terminals in the sheep brain are intensely stained.

  • Immunohistochemical detection of gamma MSH-immunoreactive neurons in the human infundibular nucleus using the GP-031-50 primary antiserum (1:40,000) and the biotinylated secondary antibody-ABC method with nickel-diaminobenzidine chromogen. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.

    Citations & References