Oxytocin, Guinea pig Polyclonal Antibody

The cryostat section of the sheep hypothalamus was incubated in guinea pig polyclonal antibodies to oxytocin at the dilution of 1:1000 overnight followed by incubation with biotinylated secondary antibodies. Cell bodies and nerve terminals (from cells that produce this hormone) in the sheep brain are intensely stained. This figure shows staining of cells when no pre-absorption is performed.

Immunofluorescent visualization of magnocellular oxytocin neurons in formalin-fixed floated cryostat section from the human paraventricular nucleus. The oxytocin antiserum (gp-037-50; 1:3,000) was reacted with Cy3-conjugated anti-guinea pig antibodies. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.

Immuno-peroxidase detection of oxytocin neurons in the human hypothalamic supraoptic nucleus, using guinea pig polyclonal oxytocin antibodies (gp-037-50; 1:50,000) with the biotinylated secondary antibody-ABC technique and Ni-DAB chromogen. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.

Immuno-peroxidase detection of oxytocin neurons in the hypothalamic paraventricular nucleus of the rat, using guinea pig polyclonal oxytocin antibodies (gp-037-50; 1:50,000) with the biotinylated secondary antibody-ABC technique and Ni-DAB chromogen. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.