Alternative NamesSecretogranin III; SgIII; SCG3; UNQ2502/PRO5990;
Application(s)IHC-Frozen, WB
Antibody HostRabbit
Antibody TypePolyclonal
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; expected to react with mouse due to sequence homology
Species ReactivityHuman, Mouse (Predicted), Rat
Immunogen DescriptionA synthetic peptide (DFINKQADAYVEK) corresponding to a region (438-450) from human Secretogranin-3. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
ConjugateUnconjugated
Purity DescriptionAffinity purified on antigen column
Application DetailsImmunohistochemistry (IHC) Western Blotting (WB) and Immunocytochemistry. A concentration of 0.1-0.5 µg/mL is recommended for WB. Human SCG3 (precursor) has a predicted length of 468 residues and MW of 53 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed frozen tissues. Heat mediated antigen retrieval is required. A concentration of 0.5-1.0 µg/mL is recommended for IHC-F. A concentration of 1.0 µg/mL is recommended for Immunocytochemistry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetSecretogranin-3 (SgIII)
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; expected to react with mouse due to sequence homology
Target Host SpeciesHuman
Species ReactivityHuman, Mouse (Predicted), Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide (DFINKQADAYVEK) corresponding to a region (438-450) from human Secretogranin-3. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
Purity DescriptionAffinity purified on antigen column
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesSecretogranin III; SgIII; SCG3; UNQ2502/PRO5990;
Scientific BackgroundSecretogranin-3 (SCG3) belongs to the chromogranin/secretogranin family of neuroendocrine secretory proteins. The protein is expressed in brain, heart, kidney, liver and skeletal muscle but the function of SCG3 is largely unknown. Genetic variations in the SCG3 gene have been suggested to influence the risk of obesity through possible regulation of hypothalamic neuropeptide secretion.
Immunohistochemical detection of Secretogranin-3 in paraffin-embedded rat brain tissue sections using Rabbit polyclonal antibody to human Secretogranin-3 (R-1081-100).