Calretinin (CR), Chicken Polyclonal Antibody

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Catalog Number
C-1801

    Product Info

  • Product Name Calretinin (CR), Chicken Polyclonal Antibody
  • Product Description google Chicken anti-Calretinin (CR) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
  • Alternative Names CR; 29 kDa calbindin;
  • Application(s) ICC, WB
  • Antibody Host Chicken
  • Antibody Type Polyclonal
  • Specificity Human, reacts with human, cow, rat, mouse. Antibody is specific for calretinin and does not recognize closely related proteins parvalbumin and calbindin as determined by Western Blotting.
  • Species Reactivity Bovine, Human, Mouse, Rat
  • Immunogen Description Full-length recombinant human protein
  • Conjugate Unconjugated
  • Purity Description IgY fraction
  • Regulatory Status For research use only.

    Specifications

  • Product Description Chicken anti-Calretinin (CR) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
  • Application(s) ICC, WB
  • Application Details Western blotting (1:1,000-1:5,000) and Immunohistochemistry (1:1,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Calretinin (CR)
  • Specificity Human, reacts with human, cow, rat, mouse. Antibody is specific for calretinin and does not recognize closely related proteins parvalbumin and calbindin as determined by Western Blotting.
  • Target Host Species Human
  • Species Reactivity Bovine, Human, Mouse, Rat
  • Antibody Host Chicken
  • Antibody Type Polyclonal
  • Antibody Isotype IgY
  • Conjugate Unconjugated
  • Immunogen Description Full-length recombinant human protein
  • Purity Description IgY fraction
  • Format Lyophilized IgY preparation, with sodium azide.
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names CR; 29 kDa calbindin;
  • Uniprot Number P22676
  • Uniprot Number/Name P22676 (CALB2_HUMAN)
  • Scientific Background Calretinin is a calcium-binding protein which is abundant in auditory neurons. Ref: uniprot.org
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • A: Adult rat brain hippocampus section stained for calretinin (red) and MeCP2 (green) by Immunohistochemistry. The chicken anti-Calretinin antibody labels a subset of hippocampal interneurons, which also express MeCP2 (R-1810-100). Co-staining is demonstrated in the nucleus by a yellow color. B: Adult rat cortex section co-stained for calretinin (red) and calbindin (M-1797-100, green). Each antibody specifically labels a subset of interneurons (i.e., calretinin-positive or calbindin-postive) that express each marker exclusively. Insets are high-magnification images of the boxed area in each picture. Blue: Nuclear DNA stained with DAPI. IHC method: 45 um sections; tissue was fixed by transcardial perfusion with 4% paraformaldehyde. C: Western blot of proteins parvalbumin (Lane 1), calretinin (Lane 2), calbindin (Lane 3) and rat brain homogenate (Lane 4) with chicken anti-calretinin antibody (1:1,000). Absence of immunreactivity with parvalbumin (Lane 1) and calbindin (Lane 3) demonstrates specificity of this antibody for calretinin (Lane 2). In rat brain, this antibody recognizes a single band at ~32 kDa, corresponding to calretinin.

  • Left: Staining of rat hippocampus with chicken anti-calretinin (green, 1:1,000) and mouse anti-parvalbumin (M-1813-100, red, 1:1,000) by Immunohistochemistry. IHC Method: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 um, and free-floating sections were stained. The calretinin and parvalbumin antibodies label different classes of interneurons. Blue: Nuclear DNA stain. Right: Western blot analysis of calretinin expression (green, 1:1,000) in rat brain (2) and spinal cord (3), mouse brain (4) and spinal cord (5), and cow spinal cord (6). A band at 29 kDa corresponds to calretinin protein. The same blot was simultaneously probed with a mouse anti-internexin antibody (red).

    Citations & References