Calbindin-binding protein, Mouse Monoclonal Antibody

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Catalog Number
M-1797

    Product Info

  • Product Name Calbindin-binding protein, Mouse Monoclonal Antibody
  • Product Description google Mouse anti-Calbindin-binding protein Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
  • Alternative Names Calbindin D28,D-28K, Vitamin D-dependent calcium-binding protein, avian-type
  • Application(s) IHC-Frozen, WB
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Specificity Human, reacts with Human, horse, Cow, Pig, chicken, Rat, Mouse.
  • Species Reactivity Bovine, Chicken, Horse, Human, Mouse, Pig, Rat
  • Immunogen Description Full-length recombinant human protein
  • Conjugate Unconjugated
  • Purity Description Protein G purified
  • Regulatory Status For research use only.

    Specifications

  • Product Description Mouse anti-Calbindin-binding protein Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
  • Application(s) IHC-Frozen, WB
  • Application Details Western blotting (1:1,000-1:5,000) and Immunohistochemistry (1:1,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Calbindin-binding protein
  • Specificity Human, reacts with Human, horse, Cow, Pig, chicken, Rat, Mouse.
  • Target Host Species Human
  • Species Reactivity Bovine, Chicken, Horse, Human, Mouse, Pig, Rat
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Antibody Isotype IgG1
  • Clone Name 4H7
  • Conjugate Unconjugated
  • Immunogen Description Full-length recombinant human protein
  • Purity Description Protein G purified
  • Format Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material.
  • Storage Instructions Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Calbindin D28,D-28K, Vitamin D-dependent calcium-binding protein, avian-type
  • Uniprot Number P05937
  • Uniprot Number/Name P05937 (CALB1_HUMAN)
  • Scientific Background Buffers cytosolic calcium. May stimulate a membrane Ca2+-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. Ref: uniprot.org
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Left and middle: Detection of calbindin immunoreactivity in rat brain cerebellum (Left) and cortex (Middle) sections by Immunohistochemistry. Calbindin (red) is predominantly expressed in the dendrites and perikarya of Purkinje cells in the molecular layer of cerebellum (left), and selectively expressed in certain type of interneurons (calbindin-postive interneuron) in the cortex (middle). Methyl-CpG binding protein 2 (Rabbit anti-MeCP2, R-1810-100, green) is universally expressed in the nuclei of almost all neurons. As a result, double-immunofluorescence shows that calbindin-expressing cell somas appear red, but the nucleus appears to be yellow. Blue: DAPI nuclear stain. Insets represent high magnification images of the boxed areas. IHC method: 45 um sections, fixed by transcardial perfusion with 4% paraformaldehyde. Right: Western blot analysis of calbindin expression in cow cerebellum homogenate. The antibody shows strong immunoractivity with one calbindin band at ~30 kDa.

  • Left: Rat brain cerebellum stained with mouse anti-calbindin (green, 1:1,000) and rabbit anti-calretinin (R-1800-50, red, 1:5,000) by Immunohistochemistry. Blue: DAPI nuclear stain. IHC Method: Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 um, and free-floating sections were stained. Calbindin antibody prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of cerebellum. In contrast, the calretinin antibody stains granule cells, in the granualar layer, and their processes in the molecular layer. Right: Western blot analysis of calbindin expression (antibody dilution: 1:2,000) in rat cerebellum (2), pig hippocampus (3), and cow cerebellum (4). The band at ~25 kDa corresponds to calbindin protein, heavily expressed in the cerebellum, but less in hippocampus.

    Citations & References