Nestin, Rabbit Polyclonal Antibody

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Catalog Number
R-1811

    Product Info

  • Product Name Nestin, Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-Nestin Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
  • Application(s) ICC, WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity Human,reacts with Human. Other species not tested.
  • Species Reactivity Human
  • Immunogen Description Part of recombinant human protein (amino acids 315-630).
  • Conjugate Unconjugated
  • Purity Description Affinity Purified
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-Nestin Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
  • Application(s) ICC, WB
  • Application Details Western blotting (1:1,000-1:5,000) and Immunocytochemistry (1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Nestin
  • Specificity Human,reacts with Human. Other species not tested.
  • Target Host Species Human
  • Species Reactivity Human
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description Part of recombinant human protein (amino acids 315-630).
  • Purity Description Affinity Purified
  • Format Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Uniprot Number P48681
  • Uniprot Number/Name P48681 (NEST_HUMAN)
  • Scientific Background Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity). Ref: uniprot.org
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Left: SH-SY5Y neuroblastoma cells stained with rabbit antibody to Nestin (red, 1:5,000) and chicken antibody to Vimentin (C-1409-50, green, 1:10,000) by Immunocytochemistry. Vimentin is main partner of nestin forming heterodimers, further polymerizing to form intermediate filaments. Co-localization of vimentin and nestin in cells is shown in yellow. Blue: DAPI staining of nuclear DNA. Right: Western blot analysis of nestin expression in SH-SY5Y cell lysate. The antibody (1:2,000) detects one specific band running at ~260 kDa corresponding to full-length nestin protein.

    Citations & References