Neuropeptide RFRP-3, Guinea pig Polyclonal Antibody

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Catalog Number
GP-1080

    Product Info

  • Product Name Neuropeptide RFRP-3, Guinea pig Polyclonal Antibody
  • Product Description google
    Guinea Pig anti-Neuropeptide RFRP-3 Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
  • Alternative Names Neuropeptide NPVF; RFRP; Neuropeptide VF; C7orf9; FMRFamide-related peptides;
  • Application(s) IHC-Frozen
  • Antibody Host Guinea Pig
  • Antibody Type Polyclonal
  • Specificity Specificity was demonstrated by immunohistochemistry. This antibody is known to react with rat and sheep.
  • Species Reactivity Human, Rat, Sheep
  • Immunogen Description A synthetic peptide (VPNLPQRF) corresponding to the amino acids 124-131 from human Neuropeptide VF. Neuropeptide VF is the precursor of the neuropeptides NPSF (RFRP-1), RFRP-2 and RFRP-3. The synthetic peptide was conjugated to a carrier protein KLH to enhance the immunological response.
  • Conjugate Unconjugated
  • Purity Description Neat serum
  • Regulatory Status For research use only.

    Specifications

  • Product Description
    Guinea Pig anti-Neuropeptide RFRP-3 Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
  • Application(s) IHC-Frozen
  • Application Details Immunohistochemistry (IHC). A concentration of 1 in 2000 is recommended. IHC performed in sheep brain (hypothalamus) demonstrates intense staining of cells and terminals. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Neuropeptide RFRP-3
  • Specificity Specificity was demonstrated by immunohistochemistry. This antibody is known to react with rat and sheep.
  • Target Host Species Human
  • Species Reactivity Human, Rat, Sheep
  • Antibody Host Guinea Pig
  • Antibody Type Polyclonal
  • Antibody Isotype Mixed
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide (VPNLPQRF) corresponding to the amino acids 124-131 from human Neuropeptide VF. Neuropeptide VF is the precursor of the neuropeptides NPSF (RFRP-1), RFRP-2 and RFRP-3. The synthetic peptide was conjugated to a carrier protein KLH to enhance the immunological response.
  • Purity Description Neat serum
  • Format Lyophilized
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions Store lyophilized antibody at 2-8ºC or lower. After reconstitution, divide into aliquots and store at -20°C, or lower, for optimal long term stability. It is recommended that a reconstituted aliquot is stored at 2-8°C for no longer than 2 weeks. Allocation of an appropriate anti-bacterial agent can increase shelf life by several weeks. Glycerol (1:1) can be added to neat serum for additional stability if intended use does not prevent this.
  • Batch Number Please see item label.
  • Expiration Date

    12 months after date of receipt (lyophilized, unopened vial).

  • Alternative Names Neuropeptide NPVF; RFRP; Neuropeptide VF; C7orf9; FMRFamide-related peptides;
  • Uniprot Number Q9HCQ7
  • Uniprot Number/Name Q9HCQ7 (NPVF_HUMAN)
  • Scientific Background Neuropeptide VF is the precursor of neuropeptides NPSF (RFRP-1), RFRP-2 and RFRP-3 (NPVF). RFRP-3 is reported to inhibit forskolin-induced production of cAMP. RFRP-3 has also been shown to block morphine-induced analgesia.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Immunoreactivity of cells and terminals in sheep hypothalamus section using anti-RFRP-3 antibody (1:2000).

  • Staining of RFRP-3 peptide is not affected by preadsorption with Kisspeptin.


    Staining of RFRP-3 peptide is not affected by preadsorption with Prolactin-releasing peptide (PrRP).


    Staining of RFRP-3 peptide is not affected by preadsorption with NFF peptide.


    Immunohistochemical detection of RFRP-3 with the guinea pig polyclonal antiserum (gp-1080-50; 1: 40,000), followed by detection with the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen, produces specific labeling of RFRP-3/GnIH neurons and their processes in the dorsomedial nucleus of the rat hypothalamus. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.

    Citations & References