SpecificityThe specificity of this antibody has been confirmed by WB against the antigen. Human; rat; predicted to react with mouse due to sequence homology;
Species ReactivityHuman, Mouse (Predicted), Rat
Immunogen DescriptionA synthetic peptide (DYDTDVSLEKIQP) corresponding to a region (425-437) from human D(1A) dopamine receptor. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
ConjugateUnconjugated
Purity DescriptionAffinity purified on antigen column
Application DetailsWestern Blotting (WB). A concentration of 1.0 µg/mL is recommended for WB. Human DRD1 has a predicted length of 446 residues and a MW of 49 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetD(1A) dopamine receptor
SpecificityThe specificity of this antibody has been confirmed by WB against the antigen. Human; rat; predicted to react with mouse due to sequence homology;
Target Host SpeciesHuman
Species ReactivityHuman, Mouse (Predicted), Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide (DYDTDVSLEKIQP) corresponding to a region (425-437) from human D(1A) dopamine receptor. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
Purity DescriptionAffinity purified on antigen column
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundD(1A) dopamine receptor (DRD1) is a multi-pass membrane protein that belongs to the G-protein coupled receptor 1 family. DRD1 is one of five receptors for dopamine. DRD1 stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. At least 2 isoforms exist arising from alternate transcription initiation sites.
Western blot analysis of rabbit polyclonal antibody to human D(1A) dopamine receptor (1 µg/ml) in rat brain tissue lysate. Secondary HRP conjugated goat anti-rabbit IgG antibody (working dilution: 1:3000-10000).