Calbindin, Mouse Monoclonal Antibody

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Catalog Number
M-976

    Product Info

  • Product Name Calbindin, Mouse Monoclonal Antibody
  • Product Description google Mouse anti-Calbindin Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
  • Alternative Names CALB1_BOVIN; CALB1;
  • Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen.
  • Species Reactivity Bovine
  • Immunogen Description Bovine kidney Calbindin-D
  • Conjugate Unconjugated
  • Purity Description IgG
  • Regulatory Status For research use only.

    Specifications

  • Product Description Mouse anti-Calbindin Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
  • Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
  • Application Details Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-1.0 µg/mL is recommended for WB. Bovine Calbindin-D has a predicted length of 261 residues and a MW of 28 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect Calbindin-D in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Calbindin
  • Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen.
  • Target Host Species Bovine
  • Species Reactivity Bovine
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Antibody Isotype IgG
  • Clone Name CB-D7
  • Conjugate Unconjugated
  • Immunogen Description Bovine kidney Calbindin-D
  • Purity Description IgG
  • Format Lyophilized from 1.2% sodium acetate, 2 mg BSA, 0.01 mg NaN3
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
  • Storage Instructions At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names CALB1_BOVIN; CALB1;
  • Uniprot Number P04467
  • Uniprot Number/Name P04467 (CALB1_BOVIN)
  • Scientific Background Calbindin-D is a highly conserved 28 kDa protein that belongs to a family of low molecular weight calcium-binding proteins (CaBPs). Calbindin-D has 4 active calcium binding domains. It plays a role in the regulation of calcium and calcium related signalling in neurons and during embryo development. This antibody binds only to calbindin-D-28K and not with other family members such as calretinin and calbindin-D-9K.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Western blot of mouse monoclonal antibody to Calbindin-D (M-976-100) using rat brain lysate (lanes 1 and 2), rat kidney lysate (lane 3) and rat lung tissue lysate (lane 4) at a concentration of 0.5 µg/mL. Secondary antibody HRP conjugated goat anti-mouse IgG at 1:3,000-10,000.

    Citations & References