Application DetailsThis antibody is recommended for WB, sandwich ELISA and immunofluorescence. The recommended dilution for this antibody is 25 µg/mL for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificitySpecificity has been confirmed by WB and direct ELISA against the antigen. Human and mouse. Other species havve not been tested.
Target Host SpeciesHuman
Species ReactivityHuman, Mouse
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG
Clone Name8C11
ConjugateUnconjugated
Immunogen DescriptionPartial recombinant human PAFAH subunit gamma (1-232) with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.2
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Cytosolic PAFAH IB is formed of three subunits, alpha (45 kDa), beta (30 kDa, and gamma (29 kDa). Its catalytic activity of PAFAH IB resides in the beta and gamma subunits while the alpha subunit has regulatory activity. Trimer formation is not essential for the catalytic activity. Specifically, PAFAH subunit gamma inactivates platelet-activating factor by removing the acetyl group at the sn-2 position. It plays an important role during the development of the brain. In the adult, it is expressed in brain, skeletal muscle, kidney, thymus, spleen, colon, testis, ovary and peripheral blood leukocytes. In the fetus, highest expression occurs in the brain.
Immunofluorescent detection of PAFAH subunit gamma expression in HeLa cells. The anti-PAFAH subunit gamma primary antibody, catalogue number M-856-100, was used at a concentration of 25 µg/mL.
Western blot detection of PAFAH subunit gamma expression in human neuroblastoma cell lysate.