Platelet-activating factor acetylhydrolase IB subunit gamma (AFAH subunit gamma), Mouse Monoclonal Antibody

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Catalog Number
M-856

    Product Info

  • Product Name Platelet-activating factor acetylhydrolase IB subunit gamma (AFAH subunit gamma), Mouse Monoclonal Antibody
  • Product Description google Mouse anti-Platelet-activating factor acetylhydrolase IB subunit gamma (AFAH subunit gamma) Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
  • Alternative Names Platelet-activating factor acetylhydrolase IB subunit gamma; PAF acetylhydrolase 29 kDa subunit; PAF-AH 29 kDa subunit; PAF-AH subunit gamma; PAFAH subunit gamma; PAFAH1B3; PAFAHG
  • Application(s) ELISA, WB
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Specificity Specificity has been confirmed by WB and direct ELISA against the antigen. Human and mouse. Other species havve not been tested.
  • Species Reactivity Human, Mouse
  • Immunogen Description Partial recombinant human PAFAH subunit gamma (1-232) with a GST tag.
  • Conjugate Unconjugated
  • Purity Description Protein G purified immunoglobulin
  • Regulatory Status For research use only.

    Specifications

  • Product Description Mouse anti-Platelet-activating factor acetylhydrolase IB subunit gamma (AFAH subunit gamma) Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
  • Application(s) ELISA, WB
  • Application Details This antibody is recommended for WB, sandwich ELISA and immunofluorescence. The recommended dilution for this antibody is 25 µg/mL for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Platelet-activating factor acetylhydrolase IB subunit gamma (AFAH subunit gamma)
  • Specificity Specificity has been confirmed by WB and direct ELISA against the antigen. Human and mouse. Other species havve not been tested.
  • Target Host Species Human
  • Species Reactivity Human, Mouse
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Antibody Isotype IgG
  • Clone Name 8C11
  • Conjugate Unconjugated
  • Immunogen Description Partial recombinant human PAFAH subunit gamma (1-232) with a GST tag.
  • Purity Description Protein G purified immunoglobulin
  • Format Lyophilized from PBS pH 7.2
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions After reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Platelet-activating factor acetylhydrolase IB subunit gamma; PAF acetylhydrolase 29 kDa subunit; PAF-AH 29 kDa subunit; PAF-AH subunit gamma; PAFAH subunit gamma; PAFAH1B3; PAFAHG
  • Uniprot Number Q15102
  • Uniprot Number/Name Q15102 (PA1B3_HUMAN)
  • Scientific Background
    Cytosolic PAFAH IB is formed of three subunits, alpha (45 kDa), beta (30 kDa, and gamma (29 kDa). Its catalytic activity of PAFAH IB resides in the beta and gamma subunits while the alpha subunit has regulatory activity. Trimer formation is not essential for the catalytic activity. Specifically, PAFAH subunit gamma inactivates platelet-activating factor by removing the acetyl group at the sn-2 position. It plays an important role during the development of the brain. In the adult, it is expressed in brain, skeletal muscle, kidney, thymus, spleen, colon, testis, ovary and peripheral blood leukocytes. In the fetus, highest expression occurs in the brain.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Immunofluorescent detection of PAFAH subunit gamma expression in HeLa cells. The anti-PAFAH subunit gamma primary antibody, catalogue number M-856-100, was used at a concentration of 25 µg/mL.

  • Western blot detection of PAFAH subunit gamma expression in human neuroblastoma cell lysate.

    Citations & References