Product DescriptionMouse anti-Sialytransferase ST8Sia II (ST8Sia II) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ELISA.
Application(s)ELISA, IHC-Frozen, WB
Application DetailsThis antibody is recommended for WB, IHC and ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetSialytransferase ST8Sia II (ST8Sia II)
SpecificitySpecificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG
Clone Name6G6
ConjugateUnconjugated
Immunogen DescriptionPartial recombinant human ST8Sia II (amino acids 40-147) with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.2
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesAlpha-2;8-sialyltransferase 8B; Sialyltransferase X; STX; ST8SIA1; SIAT8B; STX
ST8Sia II is a type II membrane protein that is thought to catalyze the transfer of sialic acid from CMP-sialic acid to N-linked oligosaccharides and glycoproteins. The encoded protein may be found in the Golgi apparatus and may be involved in the production of polysialic acid, a modulator of the adhesive properties of neural cell adhesion molecule (NCAM1). This protein is a member of glycosyltransferase family 29. It is highly expressed in fetal brain, kidney and heart and to a much lesser extent in adult heart and thymus.
Immunohistochemical detection of ST8Sia II on formalin fixed, paraffin-embedded, human prostate. Anti-ST8Sia II primary antibody, catalogue number M-874-100, was used at a concentration of 1.2 µg/mL.