Application DetailsThis antibody is recommended for WB and ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetGlutathione synthetase (GSH-S)
SpecificitySpecificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG
Clone Name5G4-2E9
ConjugateUnconjugated
Immunogen DescriptionRecombinant human GSH synthetase with GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.2
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesGlutathione synthetase; Glutathione synthase; GSH-S; GSS
Glutathione (GSH), a ubiquitous low molecular mass thiol, is important for a variety of biological functions including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. GSH synthetase acts as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamul-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency.