Application DetailsWestern Blotting (WB). A concentration of 0.5-1.0 µg/mL is recommended for WB. Rabbit TPH has a predicted length of 444 residues and a MW of 51 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetTryptophan 5-hydroxylase 1
SpecificityThe specificity of this antibody has been confirmed by WB against the antigen. Human; rat; rabbit;
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesTryptophan 5-hydroxylase 1; Tryptophan 5-monooxygenase 1; TPH1; TPH;
Scientific BackgroundTryptophan Hydroxylase (TPH) belongs to the biopterin-dependent aromatic amino acid hydroxylase family. This enzyme catalyzes the first and rate limiting step in the biosynthesis of serotonin. Mutations in this gene have been implicated in a variety of disorders including depression, aggression and schizophrenia.
Western blot using mouse monoclonal antibody to Tryptophan Hydroxylase (M-1155-100) on rat brain tissue lysate at 1 µg/ml. Secondary HRP conjugated goat anti-mouse IgG antibody at 1:3,000.