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Tyrosine hydroxylase (TH), Mouse Monoclonal Antibody
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Catalog Number
M-1616
- Product Name Tyrosine hydroxylase (TH), Mouse Monoclonal Antibody
- Product Description Mouse anti-Tyrosine hydroxylase (TH) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded, ICC, IP, FC.
- Alternative Names LNC-1; LNC1; TH monoclonal
- Application(s) FC, ICC, IHC-Frozen, IHC-Paraffin-embedded, IP, WB
- Antibody Host Mouse
- Antibody Type Monoclonal
- Specificity Clone LNC 1 recognizes an epitope on the outside of the regulatory N-terminus. The clone detects a protein of approximately 59-61 kDa by Western blot and reduced SDS-PAGE. The clone does not react with dopamine-beta-hydroxylase, phenylalanine hydroxylase, trytophan hydroxylase, dehydropteridine reductase, sepiapterin reductase or phenethanolamine-N-methyl transferase (PNMT) by western blots. Chicken, Frog, Horse, Human, Monkey, Mouse, Vole, Sheep, Zebrafish other species not yet tested
- Species Reactivity Chicken, Frog, Horse, Human, Mouse, Primate, Rat, Sheep, Vole, Zebra Fish
- Immunogen Description Tyrosine Hydroxylase purified from PC12 cells
- Conjugate Unconjugated
- Purity Description Unpurified ascites fluid, diluted with PBS containing 3% BSA, no preservatives.
- Regulatory Status For research use only.
Product Info
- Product Description Mouse anti-Tyrosine hydroxylase (TH) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded, ICC, IP, FC.
- Application(s) FC, ICC, IHC-Frozen, IHC-Paraffin-embedded, IP, WB
- Application Details Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry/paraffin embedded IH(P), Immunoprecipitation (IP), Immunofluorescence (IF), Flow cytometry (FC).WB: 1:1000 -1:2000, SDS reduced samples. Detects a 59-61kDa protein. Rat Brain lysates is a suitable control. IHC/IH(P): Reacts in formalin fixed paraffin embedded tissues with HIER antigen recovery. Typical dilution is 1:100-1:200 depending upon incubation time and detection method used. IF: 1:200-1:1000, 4% PFA fixed tissues/cells permeabilized with 0.1-0.4% triton X-100; also works in fresh frozen and acetone fixed tissues/cells.IP: 1:100, immobilized on protein A beads, Fleming-Jones et al (1995) J. Protein Chemistry 14(5):275-282.FC: Fixed, permeabilized dopaminergic nerve terminals from rat striatum, {Wolf, ME, Kapatos, G (1989) The Journal of Neuroscience, January 1989, 9(l): 108-114; Wolf ME, Zigmond, MJ, Kapatos, G (1989) J. Neurochemistry 53(3):879-885}.
- Target Tyrosine hydroxylase (TH)
- Specificity Clone LNC 1 recognizes an epitope on the outside of the regulatory N-terminus. The clone detects a protein of approximately 59-61 kDa by Western blot and reduced SDS-PAGE. The clone does not react with dopamine-beta-hydroxylase, phenylalanine hydroxylase, trytophan hydroxylase, dehydropteridine reductase, sepiapterin reductase or phenethanolamine-N-methyl transferase (PNMT) by western blots. Chicken, Frog, Horse, Human, Monkey, Mouse, Vole, Sheep, Zebrafish other species not yet tested
- Target Host Species Rat
- Species Reactivity Chicken, Frog, Horse, Human, Mouse, Primate, Rat, Sheep, Vole, Zebra Fish
- Antibody Host Mouse
- Antibody Type Monoclonal
- Antibody Isotype IgG1, kappa
- Clone Name LNC, LNC1, LNC-1
- Conjugate Unconjugated
- Immunogen Description Tyrosine Hydroxylase purified from PC12 cells
- Purity Description Unpurified ascites fluid, diluted with PBS containing 3% BSA, no preservatives.
- Format Lyophilized, dry powder.
- Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
- Storage Instructions After reconstitution keep aliquots at -20 ° to -70°C for a higher stability. At 2-8°C keep up to one week, insulated, protected from light; use sterile methods and pipettes. Highly purified glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles. Keep tightly closed when not in use and protected from light
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names LNC-1; LNC1; TH monoclonal
- Uniprot Number P04177
- Uniprot Number/Name P04177 (TY3H_RAT)
- Scientific Background Tyrosine hydroxylase is an excellent marker for dopaminergic and noradrenergic neurons. Tyrosine hydroxylase (a.k.a. tyrosine 3-monooxygenase) is the enzyme responsible for catalyzing the conversion of the amino acid L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA). L-DOPA is a precursor for dopamine, which, in turn, is a precursor for the important neurotransmitters norepinephrine (noradrenaline) and epinephrine (adrenaline). Tyrosine hydroxylase catalyzes the rate limiting step in this synthesis of catecholamines. In humans, tyrosine hydroxylase is encoded by the TH gene, and the enzyme is present in the central nervous system (CNS), peripheral symphatic neurons and the adrenal medulla. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing. Tyrosine hydroxylase, phenylalanine hydroxylase and tryptophan hydroxylase together make up the family of aromatic amino acid hydroxylases (AAAHs).
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.
Specifications
- Specific References Kapatos G., Kemski V., and Geddes T. (1989) Dopamine neurons in monolayer culture as a model system for the study of tyrosine hydroxylase, in Pteridines and Biogenic Amines in Neuropsychiatry, Pediatrics and Immunology (Levine R. A., KuhnD. M., Milstien S., and Curtius H-C., eds), pp. 243-258. Lakeshore Publishers, Grosse Pointe, Michigan.